Apoptosis Inhibitor |
Catalog No.GC10160
One-Click Copy Product Info
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A cell-permeable inhibitor of caspase-3 activation
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 54135-60-3
Sample solution is provided at 25 µL, 10mM.
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M50054, 2,2'-methylenebis (1,3-cyclohexanedione), is a novel inhibitor of apoptosis. [1]
In human Fas-expressing WC8 cells, M50054 inhibited apoptosis by soluble human Fas ligand in vitro cell death assay. M50054 inhibited the apoptotic U937 cell death which is a human monocytic leukemic cell line, induced by anticancer agents such as etoposide. M50054 inhibited apoptotic characters such as DNA phosphatidylserine and fragmentation exposure in these cells. These anti-apoptotic effects were dependent on inhibition of caspase-3 activation. Additionally, M50054 significantly inhibited anti-Fas-antibody-induced elevation of plasma aspartate aminotransferase and alanine aminotransferase. Alopecia (hair loss) symptoms were also significantly improved with topical treatment with M50054. M50054 inhibits apoptosis induced by a variety of stimuli via inhibition of caspase-3 activation, and may thus be effective for hepatitis and chemotherapy-induced alopecia. [1]
Reference:
[1].Tsuda T, Ohmori Y, Muramatsu H et al. Inhibitory effect of M50054, a novel inhibitor of apoptosis, on anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia.Eur J Pharmacol. 2001 Dec 14;433(1):37-45.
| Cas No. | 54135-60-3 | SDF | |
| Chemical Name | 2-[(2,6-dioxocyclohexyl)methyl]cyclohexane-1,3-dione | ||
| Canonical SMILES | C1CC(=O)C(C(=O)C1)CC2C(=O)CCCC2=O | ||
| Formula | C13H16O4 | M.Wt | 236.3 |
| Solubility | ≥ 9.1mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.2319 mL | 21.1595 mL | 42.3191 mL |
| 5 mM | 846.4 μL | 4.2319 mL | 8.4638 mL |
| 10 mM | 423.2 μL | 2.116 mL | 4.2319 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)