BCA Protein Assay Kit |
Catalog No.GK10009 |
El Kit de Ensayo de Proteína BCA es un reactivo listo para usar, compatible con detergentes y relacionado con Western blot que se utiliza para la determinación rápida de la concentración total de proteínas en una muestra.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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The BCA Protein Assay Kit is a ready-to-use total protein analysis reagent that can quickly determine total protein concentration by measuring the absorbance at 562 nm and comparing it with the absorbance-concentration curve of a protein standard.
This kit is suitable for measuring the total protein content of purified proteins, animal and plant tissue samples, and various cell samples. The detection concentration range is 20-2000 μg/mL. As the protein concentration increases, the absorbance has a linear relationship with the protein concentration.
This kit is developed based on the BCA (Bicin-choninic Acid) method, which enables fast, stable and sensitive concentration determination of protein. The principle is that under alkaline conditions, the protein reduces Cu2+ to Cu+, and Cu+ forms a purple complex with the BCA reagent. This water-soluble complex shows strong absorbance at 562 nm.
If you need to accurately quantify the protein to be tested, it is recommended to choose a protein standard with a quality similar to that of the protein to be tested. For example, when measuring immunoglobulin samples, bovine gamma globulin (BGG) standards can be used.
In addition, this kit provides a series of concentration gradient BSA (Bovine Serum Albumin, bovine serum albumin) standards with a concentration range of 150-2000 μg/mL, eliminating the need for a series of gradient dilution processes and making the detection process faster.
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