Acute pancreatitis (AP) is a common clinical acute abdomen with complex etiology, with a mortality rate of 5% to 10%, and severe AP mortality as high as 20% to 30%. Way. At present, the commonly used methods for making in vivo AP animal models include pancreatic duct ligation, duodenal loop closure, pancreaticobiliary puncture and injection, pancreatic subcapsular injection, pancreaticobiliary injection combined with intravenous infusion, and bombesin in combination. Intraperitoneal injection of lipopolysaccharide, etc.

We all know that there are many types of fluorescent dyes, such as: Cyanines, SIR, Fluorescein, TAMRA, ICG, etc. Today, I will show you one of them, Cyanines. For details, please see the following:

There are two types of cyanine dyes: non-sulfonated cyanines and sulfonated cyanines. For many applications, they are interchangeable because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used to label biomolecules such as DNA and proteins. The difference between dyes is their solubility: sulfur dyes are water soluble, and they do not use organic co-solvents for marking in aqueous environments. They do not tend to aggregate in water. In some cases, one type of cyanine is required.

Streptococcus pyogenes, also known as group A streptococcus (GAS), causes a wide variety of acute infections, ranging from localized purulent infections to severe, even fatal, invasive disease. Systemic spread is usually caused by bacterial penetration of the epithelial barrier of the pharynx or damaged skin and, if not well controlled, can lead to blood and soft tissue invasion. Superficial colonization and invasive infection of GAS depend on secreted GAS virulence factors, of which cysteine protease exotoxin (SpeB) is the key. SpeB is initially an inactive zymogen that is proteolytically converted to a mature catalytically active enzyme. SpeB contributes to epidermal localization and systemic spread, but the underlying mechanisms are unknown.