Clonidine-d4 (hydrochloride) |
Catalog No.GC47103
One-Click Copy Product Info
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An internal standard for the quantification of clonidine
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 67151-02-4
Sample solution is provided at 25 µL, 10mM.
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Clonidine-d4 is intended for use as an internal standard for the quantification of clonidine by GC- or LC-MS. Clonidine is an agonist of α2-adrenergic receptors (α2-ARs; Kis = 61.66, 69.18, and 134.9 nM for α2A-, α2B-, and α2C-ARs, respectively).1 It stimulates [35S]GTPγS binding to HEK293 cell membranes expressing the human receptors with EC50 values of 26.92, 56.23, and 912.01 nM for α2A-, α2B-, and α2C-ARs, respectively. Clonidine also binds to I1-imidazoline sites in a variety of cell and tissue types (Kds = 4-15 nM).2 It induces relaxation of isolated mesenteric artery rings precontracted with norepinephrine when used at a concentration of 10 μM.3 Clonidine (10 μM) also induces membrane hyperpolarization and reduces norepinephrine-induced depolarization in isolated mesenteric artery rings. Clonidine (0.1 and 1 μg/kg) reduces mean blood pressure and heart rate when administered via microinjection to the nucleus reticularis lateralis (NRL) of anesthetized normotensive cats.4 Formulations containing clonidine have been used in the treatment of hypertension.
1.Jasper, J.R., Lesnick, J.D., Chang, L.K., et al.Ligand efficacy and potency at recombinant α2 adrenergic receptors: Agonist-mediated [35S]GTPγS bindingBiochem. Pharmacol.55(7)1035-1043(1998) 2.Ernsberger, P., Graves, M.E., Graff, L.M., et al.I1-imidazoline receptors. Definition, characterization, distribution, and transmembrane signalingAnn. N.Y. Acad. Sci. 763(1)22-42(1995) 3.Silva, E.G., Feres, T., Vianna, L.M., et al.Dual effect of clonidine on mesenteric artery adrenoceptors: Agonistic (alpha-2) and antagonistic (alpha-1)J. Pharmacol. Exp. Ther.277(2)872-876(1996) 4.Bousquet, P., Feldman, J., and Schwartz, J.Central cardiovascular effects of alpha adrenergic drugs: Differences between catecholamines and imidazolinesJ. Pharmacol. Exp. Ther.2301(1)232-236(1984)
| Cas No. | 67151-02-4 | SDF | |
| Canonical SMILES | ClC1=C(NC2=NC([2H])([2H])C([2H])([2H])N2)C(Cl)=CC=C1.Cl | ||
| Formula | C9H5Cl2D4N3.HCl | M.Wt | 270.6 |
| Solubility | DMSO: slightly soluble,Methanol: slightly soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6955 mL | 18.4775 mL | 36.9549 mL |
| 5 mM | 739.1 μL | 3.6955 mL | 7.391 mL |
| 10 mM | 369.5 μL | 1.8477 mL | 3.6955 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)