Eugenol-d3 |
Catalog No.GC66444
One-Click Copy Product Info
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Eugenol-d3 es un derivado triplemente deuterado (d3) derivative of the common natural product eugenoldel producto natural común eugenol
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1335401-17-6
Sample solution is provided at 25 µL, 10mM.
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Eugenol-d3 es un derivado triplemente deuterado (d3) derivative of the common natural product eugenoldel producto natural común eugenol [1]. Eugenol es un aceite esencial que se encuentra en los clavos que tiene actividades antimicrobians, repelentes de insectos y antioxidantes [2-3].
References:
[1]. Zhao DH, Ke CL, Liu Q, et al. Elimination kinetics of eugenol in grass carp in a simulated transportation setting. BMC veterinary research. 2017 Dec; 13: 1-6.
[2]. Marchese A, Barbieri R, Coppo E, et al. Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint. Critical reviews in microbiology. 2017 Nov 2; 43(6): 668-689.
[3]. Ulanowska M, Olas B. Biological properties and prospects for the application of eugenol—a review. International journal of molecular sciences. 2021 Apr 1; 22(7): 3671.
Este protocolo utiliza Eugenol-d3 como estándar interno para la cuantificación de Eugenol en muestras biológicas o químicas utilizando GC-MS. Las condiciones reales de aplicación pueden requerir optimización dependiendo de la complejidad de la matriz y la sensibilidad del instrumento [1-2].
1. Preparación de reactivos y matriales
Eugenol-d3 (≥98% de pureza isotópica), estándar de Eugenol, disolvente de extracción, matriz de muestra, sistema GC-MS.
2. Pasos experimentales
(1) Preparación de la norma interna
Disuelva Eugenol-d3 en metanol para preparar una solución de caldo. Almacenar a -20℃, proteger de la luz.
(2) Picking y extracción de muestras
Agregue un volumen definido de solución de Eugenol-d3 a cada muestra antes de la extracción. Agregue disolvente de extracción, vórtice y centrífuga para recoger el sobrenadante.
(3) Análisis de instrumentos
Inyectar extracto en GC-MS. Monitoree las transiciones de masa específicas (m/z 164→134 para Eugenol, m/z 167→137 para Eugenol-d3). Calcule la concentración de analito utilizando una curva de calibración construida con una concentración estándar interna fija.
Notas y precauciones
(1) A segúrese de que la concentración de Eugenol-d3 esté dentro del rango lineal del método.
(2) El estándar interno debe agregarse antes de la extracción para corregir los efectos de la matriz y la pérdida.
(3) Mantenga el volumen de inyección y el procedimiento de preparación de muestras consistentes para mantener la calidad de los datos.
References:
[1]. Zhao DH, Ke CL, Liu Q, et al. Elimination kinetics of eugenol in grass carp in a simulated transportation setting. BMC veterinary research. 2017 Dec; 13: 1-6.
[2]. Liu H, Li J, Wang C. Development of a SID-SPE-GC-MS/MS isotope dilution assay for the quantification of eugenol in water samples. Aquaculture Research. 2018 Jan; 49(1): 582-585.
| Cas No. | 1335401-17-6 | SDF | |
| Formula | C10H9D3O2 | M.Wt | 167.22 |
| Solubility | DMSO : 100 mg/mL (598.01 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.9801 mL | 29.9007 mL | 59.8015 mL |
| 5 mM | 1.196 mL | 5.9801 mL | 11.9603 mL |
| 10 mM | 598 μL | 2.9901 mL | 5.9801 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)