Eugenol-d3 |
Catalog No.GC66444
One-Click Copy Product Info
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Eugenol-d3 is a triply deuterated (d3) derivative of the common natural product eugenol.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1335401-17-6
Sample solution is provided at 25 µL, 10mM.
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Eugenol-d3 is a triply deuterated (d3) derivative of the common natural product eugenol [1]. Eugenol is an essential oil found in cloves that has antimicrobial, insect repellent and antioxidant activities [2-3].
References:
[1]. Zhao DH, Ke CL, Liu Q, et al. Elimination kinetics of eugenol in grass carp in a simulated transportation setting. BMC veterinary research. 2017 Dec; 13: 1-6.
[2]. Marchese A, Barbieri R, Coppo E, et al. Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint. Critical reviews in microbiology. 2017 Nov 2; 43(6): 668-689.
[3]. Ulanowska M, Olas B. Biological properties and prospects for the application of eugenol—a review. International journal of molecular sciences. 2021 Apr 1; 22(7): 3671.
This protocol uses Eugenol-d3 as an internal standard for the quantification of Eugenol in biological or chemical samples using GC-MS. Actual application conditions may require optimization depending on matrix complexity and instrument sensitivity [1-2].
1. Preparation of Reagents and Materials
Eugenol-d3 (≥98% isotopic purity), Eugenol standard, extraction solvent, sample matrix, GC-MS system.
2. Experimental Steps
(1) Internal Standard Preparation
Dissolve Eugenol-d3 in methanol to prepare a stock solution. Store at -20℃, protect from light.
(2) Sample Spiking and Extraction
Add a defined volume of Eugenol-d3 solution to each sample before extraction. Add extraction solvent, vortex, and centrifuge to collect supernatant.
(3) Instrument Analysis
Inject extract into GC-MS. Monitor specific mass transitions (m/z 164→134 for Eugenol, m/z 167→137 for Eugenol-d3). Calculate analyte concentration using a calibration curve constructed with fixed internal standard concentration.
Notes and Precautions
(1) Ensure the concentration of Eugenol-d3 falls within the linear range of the method.
(2) Internal standard must be added before extraction to correct for matrix effects and loss.
(3) Keep consistent injection volume and sample preparation procedure to maintain data quality.
References:
[1]. Zhao DH, Ke CL, Liu Q, et al. Elimination kinetics of eugenol in grass carp in a simulated transportation setting. BMC veterinary research. 2017 Dec; 13: 1-6.
[2]. Liu H, Li J, Wang C. Development of a SID-SPE-GC-MS/MS isotope dilution assay for the quantification of eugenol in water samples. Aquaculture Research. 2018 Jan; 49(1): 582-585.
| Cas No. | 1335401-17-6 | SDF | |
| Formula | C10H9D3O2 | M.Wt | 167.22 |
| Solubility | DMSO : 100 mg/mL (598.01 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.9801 mL | 29.9007 mL | 59.8015 mL |
| 5 mM | 1.196 mL | 5.9801 mL | 11.9603 mL |
| 10 mM | 598 μL | 2.9901 mL | 5.9801 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)