Harmalol (hydrochloride hydrate) (Synonyms: 11-hydroxy Harmalan, Harmidol) |
Catalog No.GC49855
One-Click Copy Product Info
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A β-carboline alkaloid and an active metabolite of harmaline
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6028-00-8
Sample solution is provided at 25 µL, 10mM.
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Harmalol is a β-carboline alkaloid and an active metabolite of harmaline that has been found in P. harmala and has diverse biological activities.1,2,3,4,5 It is an inhibitor of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A; IC50 = 0.63 µM) and monoamine oxidase A (MAO-A; IC50 = 0.66 µM).3 It is selective for DYRK1A over Cdk1, Cdk5, CK1α1, Clk4, DYRK2, Pim-1, and GSK3β but also inhibits DYRK1B and Clk1 at 10 µM. It inhibits proliferation of H4 human glioblastoma cells (IC50s = 23.7 µM). Harmalol (0.5-12.5 µM) reduces increases in the levels of the cytochrome P450 (CYP) isoform CYP1A1 induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in HepG2 cells and prevents TCDD-induced activation of the aryl hydrocarbon receptor (AhR) in guinea pig hepatic cytosolic extracts.4 It reduces glutamate-induced cytotoxicity, cytochrome c release, caspase-3 activation, and the production of reactive oxygen species (ROS) in PC12 cells when used at a concentration of 25 µM.5 This product is also available as an analytical reference standard .
1.Brierley, D.I., and Davidson, C.Developments in harmine pharmacology - implications for ayahuasca use and drug-dependence treatmentProg. Neuropsychopharmacol. Biol. Psychiatry39(2)263-272(2012) 2.Nikam, T.D., Nitnaware, K.M., and Ahire, M.L.Alkaloids derived from tryptophan: Harmine and related alkaloidsNatural products. Phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes553-574(2013) 3.Tarpley, M., Oladapo, H.O., Strepay, D., et al.Identification of harmine and β-carboline analogs from a high-throughput screen of an approved drug collection; profiling as differential inhibitors of DYRK1A and monoamine oxidase A and for in vitro and in vivo anti-cancer studiesEur. J. Pharm. Sci.162105821(2021) 4.El Gendy, M.A.M., Soshilov, A.A., Denison, M.S., et al.Harmaline and harmalol inhibit the carcinogen-activating enzyme CYP1A1 via transcriptional and posttranslational mechanismsFood Chem. Toxicol.50(2)353-362(2012) 5.Han, E.S., and Lee, C.S.Inhibition of glutamate-induced change in mitochondrial membrane permeability in PC12 cells by 1-methylated β-carbolinesBiomol. Ther. (Seoul)11(2)112-118(2003)
| Cas No. | 6028-00-8 | SDF | |
| Sinónimos | 11-hydroxy Harmalan, Harmidol | ||
| Canonical SMILES | OC1=CC=C2C(NC3=C2CCN=C3C)=C1.Cl.O.O | ||
| Formula | C12H12N2O • HCl [2H2O] | M.Wt | 272.7 |
| Solubility | DMF: 10 mg/ml,DMSO: 1 mg/ml,Ethanol: 2 mg/ml,PBS (pH 7.2): insol | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.667 mL | 18.3352 mL | 36.6703 mL |
| 5 mM | 733.4 μL | 3.667 mL | 7.3341 mL |
| 10 mM | 366.7 μL | 1.8335 mL | 3.667 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)