Histone H3 (Citrullinated R26) (21-44)-GGK-biotin Peptide (trifluoroacetate salt) (Synonyms: ATKAA-Cit-KSAPATGGVKKPHRYRPG-GGK(Biotin), H3R26Cit, Histone H3 (21-44) (Arg26cit), Cit26-Histone H3 (21-44)-GGK(Biotin)) |
Catalog No.GC52479
One-Click Copy Product Info
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A biotinylated peptide fragment of histone H3
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Histone H3 (citrullinated R26) (21-44)-GGK-biotin is a peptide fragment of histone H3 that corresponds to amino acid residues 22-45 of the human H3.1 and H3.2 sequences. It is citrullinated at arginine 26 and biotinylated via a C-terminal GGK linker. Citrullination of histone H3 at arginine 26 occurs via deimination of the arginine residue by protein arginine deiminase 2 (PAD2) or PAD4 and is associated with transcriptional activation.1,2,3 It inhibits methylation of H3K27 and is elevated in bone marrow mesenchymal stem cells (BMMSCs) derived from patients with monoclonal gammopathy of undetermined significance (MGUS) or multiple myeloma.1,2
1.Clancy, K.W., Russell, A.-M., Subramanian, V., et al.Citrullination/methylation crosstalk on histone H3 regulates ER-target gene transcriptionACS Chem. Biol.12(6)1691-1702(2017) 2.McNee, G., Eales, K.L., Wei, W., et al.Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myelomaLeukemia31(2)373-381(2017) 3.Fuhrmann, J., and Thompson, P.R.Protein arginine methylation and citrullination in epigenetic regulationACS Chem. Biol.11(3)654-668(2016)
| Cas No. | SDF | ||
| Sinónimos | ATKAA-Cit-KSAPATGGVKKPHRYRPG-GGK(Biotin), H3R26Cit, Histone H3 (21-44) (Arg26cit), Cit26-Histone H3 (21-44)-GGK(Biotin) | ||
| Canonical SMILES | O=C(O)C(F)(F)F.O=C(CCCC[C@@H]1SC[C@@]([H])([C@@]1(N2)[H])NC2=O)NCCCC[C@H](NC(CNC(CNC(CNC([C@@H]3CCCN3C([C@@H](NC([C@H](CC4=CC=C(C=C4)O)NC([C@@H](NC([C@H](CC5=CN=CN5)NC([C@@H]6CCCN6C([C@@H](NC([C@@H](NC([C@@H](NC(CNC(CNC([C@@]([H])(NC([C@H](C)NC([C@@H]7CCCN7C([C@H](C)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](C)NC([C@H](C)NC([C@@H](NC([C@@]([H])(NC([C@H](C)N)=O)[C@H](O)C)=O)CCCCN)=O)=O)=O)CCCNC(N)=O)=O)CCCCN)=O)CO)=O)=O)=O)=O)[C@H](O)C)=O)=O)=O)C(C)C)=O)CCCCN)=O)CCCCN)=O)=O)=O)CCCNC(N)=N)=O)=O)CCCNC(N)=N)=O)=O)=O)=O)=O)C(O)=O | ||
| Formula | C129H216N44O35S ? XCF3COOH | M.Wt | 2975.5 |
| Solubility | Water: 1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 336.1 μL | 1.6804 mL | 3.3608 mL |
| 5 mM | 67.2 μL | 336.1 μL | 672.2 μL |
| 10 mM | 33.6 μL | 168 μL | 336.1 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)