MFI8 |
| Catalog No.GC65989 |
MFI8 es un inhibidor de molécula pequeÑa de la fusiÓn mitocondrial.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 694488-83-0
Sample solution is provided at 25 µL, 10mM.
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MFI8 is a small molecule inhibitor of mitochondrial fusion[1].
MFI8 (20 μM; 6 h) reduces significantly mitochondrial aspect ratio with an EC50 of 4.8 μM, and inhibits mitochondrial fusion via mitofusins and subsequently promoting mitochondrial fission[1].
MFI8 possesses functional groups that meet the criteria of the pharmacophore model to interact with mitofusins and promotes mitochondrial fission[1].
MFI8 binds to the HR2 domain of MFN2, modulates MFN conformation and complexes, and alters mitochondrial functionality[1].
MFI8 (0-20 μM; 6 h) concentration-responsively increases caspase-3/7 activity, induces cytochrome c release and decreases membrane potential in a mitofusin-dependent manner. MFI8 also induces DNA damage and induces cell death in combination with BV6 SMAC (second mitochondria-derived activator of caspases) mimetic[1].
| Cas No. | 694488-83-0 | SDF | |
| Formula | C16H18ClNO | M.Wt | 275.77 |
| Solubility | DMSO : 100 mg/mL (362.62 mM; Need ultrasonic) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6262 mL | 18.1311 mL | 36.2621 mL |
| 5 mM | 0.7252 mL | 3.6262 mL | 7.2524 mL |
| 10 mM | 0.3626 mL | 1.8131 mL | 3.6262 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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