X1 compound |
Catalog No.GC26082
One-Click Copy Product Info
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X1 compound es un aglutinante de alta afinidad al motivo RepA del prototipo de RNA no codificante Xist con un Kd de 0.4±0.3μM.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
X1 compound es un aglutinante de alta afinidad al motivo RepA del prototipo de RNA no codificante Xist con un Kd de 0.4±0.3μM. X1 compound muestra efectos progresivamente disruptivos en las interaccioness RepA-PRC2 y RepA-SPEN, con valores IC50 de 30μM y 48μM, respectivamente, y bloquea el inicio de la inactivación de X-chromosome inactivation. X1 compound exhibe efectos inhibitorios en el crecimiento celular y la diferenciación a una concentración de 5μM[1].
In vitro, los cuerpos embrionarios femeninos (EB) tratados por 10 μM X1 compoundderivados de células ES de ratón con dos X chromosomas durante 1-5 días inhibiron la diferenciación y el crecimiento celular, con la inhibición de la interacción RepA-PRC2 y RepA-SPEN observada en el día 2, y el pobre crecimiento de EB, el enriquecimiento contundente de H3K27me3 y la inactivación de X-chromosome observada después de 72 horas[1].
References:
[1] Aguilar R, Spencer KB, Kesner B,et al. Targeting Xist with compounds that disrupt RNA structure and X inactivation. Nature. 2022 Apr;604(7904):160-166
| Experimentos celulares [1]: | |
Líneas celulares | Células madre embrionarias de ratón |
Método de preparación | El modelo de célula del tronco embrionario hembra(ES) del ratón(TsixTST/+) inicialmente lleva dos X chromosoma activos (Xa), pero recapitula la inactivación de X-chromosome cuando se induce para diferenciarse en cuerpos embrioides (EB) durante 4 días. El proceso de diferenciación se llevó a cabo con 10μM compound X1 el día 2 , luego la exposición se prolongó hasta el día 7. |
Condiciones de reacción | 10µM; 1-5 días |
Áreas de aplicación | El tratamiento con X1 compound resultó en una inhibición específica de las mujeres de la diferenciación y el crecimiento celular al reducir la interacción RepA–PRC2 y RepA-SPEN y bloquear el inicio de la inactivación de X-chromosome. |
References: | |
| Cas No. | SDF | ||
| Formula | C27H20N4O | M.Wt | 416.48 |
| Solubility | DMSO: 83 mg/mL (199.29 mM);Water: Insoluble;Ethanol: 20 mg/mL (48.02 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4011 mL | 12.0054 mL | 24.0108 mL |
| 5 mM | 480.2 μL | 2.4011 mL | 4.8022 mL |
| 10 mM | 240.1 μL | 1.2005 mL | 2.4011 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)