4-MUNANA (sodium salt) (Synonyms: 4-MUNANA, Neu5Ac-α-4MU, Sodium 2-(4-methylumbelliferyl)-N-acetylneuraminate, 4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid) |
رقم الكتالوجGC42448
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4-Methylumbelliferyl-N-acetyl-α ؛ -D-Neuraminic Acid (ملح الصوديوم) عبارة عن ركيزة فلورية تستخدم لفحص نشاط النورامينيداز.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 76204-02-9
Sample solution is provided at 25 µL, 10mM.
4-MUNANA (sodium salt) is a fluorescent substrate for neuraminidase activity assays[1]. When 4-MUNANA is hydrolyzed by the enzyme, it releases the fluorophore 4-methylumbelliferyl (4-MU), which has an excitation maximum at 365nm and an emission maximum at 450nm[2, 3]. 4-MUNANA can be used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus[4]. 4-MUNANA can be used to assay sialidase activity[5].
References:
[1]Feng C, Li J, Snyder G, et al. Antibody against microbial neuraminidases recognizes human sialidase 3 (NEU3): the neuraminidase/sialidase superfamily revisited[J]. MBio, 2017, 8(3): 10.1128/mbio. 00078-17.
[2] Singleton E V, Gates C J, David S C, et al. Enhanced immunogenicity of a whole-inactivated influenza A virus vaccine using optimised irradiation conditions[J]. Frontiers in Immunology, 2021, 12: 761632.
[3]Naik N G, Lo Y W, Wu T Y, et al. Baculovirus as an efficient vector for gene delivery into mosquitoes[J]. Scientific reports, 2018, 8(1): 17778.
[4] Wetherall N T, Trivedi T, Zeller J, et al. Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network[J]. Journal of Clinical Microbiology, 2003, 41(2): 742-750.
[5] Seol B, Kim Y D, Cho Y S. Modeling sialidosis with neural precursor cells derived from patient-derived induced pluripotent stem cells[J]. International Journal of Molecular Sciences, 2021, 22(9): 4386.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Stock solution: Equilibrate the cryopreserved solid to room temperature, dissolve 4-MUNANA in the assay buffer, and prepare a 1mM stock solution.
Note: After aliquoting, store the unused stock solution at -20°C or -80°C away from light to avoid repeated freezing and thawing.
(2) Working solution: Dilute the stock solution with the assay buffer to the required working concentration, for example, 100μM.
Note: Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration yourself. The working solution must be prepared and used immediately.
2. Determination of Neuraminidase activity using 4-MUNANA[1] (from the literature, for reference only)
(1) Mix 5μg of total cell protein with 30μL of 4-MUNANA (final concentration of 100μM) in a 96-well plate and incubate at 37 °C for 1h.
(2) Add 150μL of 0.14M NaOH (dissolved in 83% ethanol) solution to terminate the reaction.
(3) Measure the fluorescence signal at 365nm and 450nm using a multi-functional microplate reader.
3. Measure Sialidase activity using 4-MUNANA[2] (from the literature, for reference only)
(1) Cells were suspended in assay buffer (20mM citrate (pH 4.5), 60mM NaCl and 1mM MgCl2) and lysed by ultrasonic treatment.
(2) 2μg of cell lysate was added to 100μL assay buffer (containing 100μM 4-MUNANA) and incubated at 37 °C for 4h.
(3) Terminate the reaction by adding 50μL 0.1M glycine-NaOH (pH 10.5) buffer.
(4) Measure the fluorescence signal at 365nm and 450nm using a multi-functional microplate reader.
Note: This protocol provides guidance for measuring Neuraminidase and Sialidase activities using 4-MUNANA. It can be adjusted according to other literature and specific experimental requirements.
References:
[1]Naik N G, Lo Y W, Wu T Y, et al. Baculovirus as an efficient vector for gene delivery into mosquitoes[J]. Scientific reports, 2018, 8(1): 17778.
[2]Seol B, Kim Y D, Cho Y S. Modeling sialidosis with neural precursor cells derived from patient-derived induced pluripotent stem cells[J]. International Journal of Molecular Sciences, 2021, 22(9): 4386.
| Cas No. | 76204-02-9 | SDF | |
| المرادفات | 4-MUNANA, Neu5Ac-α-4MU, Sodium 2-(4-methylumbelliferyl)-N-acetylneuraminate, 4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid | ||
| Canonical SMILES | O=C1C=C(C)C2=CC=C(O[C@]3(C([O-])=O)C[C@H](O)[C@@H](NC(C)=O)[C@@]([C@H](O)[C@H](O)CO)([H])O3)C=C2O1.[Na+] | ||
| Formula | C21H24NO11•Na | M.Wt | 489.4 |
| الذوبان | DMF: 15 mg/ml,DMSO: 20 mg/ml,Ethanol: 0.25 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0433 mL | 10.2166 mL | 20.4332 mL |
| 5 mM | 408.7 μL | 2.0433 mL | 4.0866 mL |
| 10 mM | 204.3 μL | 1.0217 mL | 2.0433 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 17 reference(s) in Google Scholar.)