Ac-Pro-Gly-Pro-OH |
رقم الكتالوجGA20643
One-Click Copy Product Info
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Ac-Pro-Gly-Pro-OH is an endogenous degradation product of extracellular collagen and acts as a CXCR2 agonist.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 292171-04-1
Sample solution is provided at 25 µL, 10mM.
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Ac-Pro-Gly-Pro-OH is an endogenous degradation product of extracellular collagen and acts as a CXCR2 agonist. Ac-Pro-Gly-Pro-OH can be used in research related to sepsis, chronic obstructive pulmonary disease, cystic fibrosis, bronchiolitis obliterans syndrome, severe asthma, idiopathic pulmonary fibrosis, and corneal ulcers[1-4].
In vitro, human endothelial progenitor cells (hEPCs) were treated with Ac-Pro-Gly-Pro-OH (0.1-1μM) for 12-24 hours. Ac-Pro-Gly-Pro-OH significantly increased the migration, tube formation, and proliferation of hEPCs, and these effects were dependent on CXCR2[5]. Cortical neurons were treated with Ac-Pro-Gly-Pro-OH (0.01-100nM) for 24 hours. Ac-Pro-Gly-Pro-OH significantly induced neuronal apoptosis, activated ERK1/2, and led to caspase-3 cleavage[6].
In vivo, Ac-Pro-Gly-Pro-OH (1μM; 20μl; injected at 4 sites; once daily) was intramuscularly administered to a mouse model of ischemic hindlimb for 4 weeks. Ac-Pro-Gly-Pro-OH significantly improved blood perfusion, reduced tissue necrosis, increased the density of CD31+ capillaries and α-SMA+ arteries, and promoted the mobilization of circulating angiogenic cells (CACs)[7]. Ac-Pro-Gly-Pro-OH (10mg/kg/day) was injected intraperitoneally into C57BL/6J mice with gallstone models for four weeks. Ac‑Pro‑Gly‑Pro‑OH enhanced CXCR2 activity, promoted neutrophil extracellular trap (NET) formation, and significantly increased gallstone formation in diabetic mice fed a lithogenic diet[8].
References:
[1] Kim SD, Lee HY, Shim JW, et al. Activation of CXCR2 by extracellular matrix degradation product acetylated Pro-Gly-Pro has therapeutic effects against sepsis. Am J Respir Crit Care Med. 2011 Jul 15;184(2):243-51.
[2] Patel DF, Snelgrove RJ. The multifaceted roles of the matrikine Pro-Gly-Pro in pulmonary health and disease. Eur Respir Rev. 2018 Jun 27;27(148):180017.
[3] Lee YC, Jackson PL, Jablonsky MJ, et al. NMR conformational analysis of cis and trans proline isomers in the neutrophil chemoattractant, N-acetyl-proline-glycine-proline. Biopolymers. 2001 May;58(6):548-61.
[4] Wu JW, Zhou YT, Wang BX, et al. Network Pharmacology-Based and Experimental Validation Elucidate the Target Mechanism of Vinorine in Ameliorating Secondary Brain Injury After Intracerebral Hemorrhage. CNS Neurosci Ther. 2025 Sep;31(9):e70609.
[5] Kwon YW, Heo SC, Lee TW, et al. N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells. Sci Rep. 2017 Feb 23;7:43057.
[6] Hill JW, Nemoto EM. Matrix-derived inflammatory mediator N-acetyl proline-glycine-proline is neurotoxic and upregulated in brain after ischemic stroke. J Neuroinflammation. 2015 Nov 21;12:214.
[7] Kwon YW, Lee SJ, Heo SC, et al. Role of CXCR2 in the Ac-PGP-Induced Mobilization of Circulating Angiogenic Cells and its Therapeutic Implications. Stem Cells Transl Med. 2019 Mar;8(3):236-246.
[8] Shi C, Feng S, Liu T, et al. Diabetes Mellitus Facilitates Gallstone Formation Through CXCR2-NETs-Mediated Liver-Bile Barrier Damage. Adv Sci (Weinh). 2026 Apr;13(24):e19500.
| Cell experiment [1]: | |
Cell lines | human endothelial progenitor cells (hEPCs) |
Preparation Method | hEPCs were maintained in endothelial cell basal medium supplemented with 5% fetal bovine serum (FBS), human VEGF, human fibroblast growth factor-2, human epidermal growth factor, insulin-like growth factor-1, and ascorbic acid at 37°C, 5% CO₂. For migration and tube formation assays, hEPCs were harvested, resuspended in EBM-2, and then treated with Ac-Pro-Gly-Pro-OH at concentrations of 0.1–1μM for 12 hours. For proliferation assay, hEPCs were seeded in gelatin-coated plates, starved in EBM-2 containing 0.5% FBS, and then treated with 0.1μM Ac-PGP for 24 hours. |
Reaction Conditions | 0.1-1μM; 12-24h |
Applications | Pro-Gly-Pro-OH significantly increased migration, tube formation, and proliferation of hEPCs. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | Mice were fed a lithogenic diet (LD; containing 1.25% cholesterol, 15% total fat, and 0.5% cholic acid) for four weeks to induce gallstone formation. Starting from the beginning of the diet, mice received Ac‑Pro‑Gly‑Pro‑OH at a dose of 10mg/kg via intraperitoneal injection once daily for four consecutive weeks. |
Dosage form | 10mg/kg; i.p.; once daily for four weeks |
Applications | Ac‑Pro‑Gly‑Pro‑OH enhanced CXCR2 activity, promoted neutrophil extracellular trap (NET) formation, and significantly increased gallstone formation in diabetic mice fed a lithogenic diet. |
References: | |
| Cas No. | 292171-04-1 | SDF | |
| Formula | C14H21N3O5 | M.Wt | 311.34 |
| الذوبان | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2119 mL | 16.0596 mL | 32.1192 mL |
| 5 mM | 642.4 μL | 3.2119 mL | 6.4238 mL |
| 10 mM | 321.2 μL | 1.606 mL | 3.2119 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)