Tetramethylrhodamine methyl ester (perchlorate) (Synonyms: TMRM, TMR methyl ester) |
رقم الكتالوجGC19476 |
رباعي ميثيل رودامين ميثيل إستر (بيركلورات) هو صبغة فلورية حمراء محبة للدهون من الخلايا دائمة الخضرة (& لامدا ؛ مثال = 530 نانومتر ، & لامدا ؛ em = 592 نانومتر).
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Cas No.: 115532-50-8
Sample solution is provided at 25 µL, 10mM.
Tetramethylrhodamine methyl ester (perchlorate) is the perchlorate form of the cell-permeable cationic red fluorescent probe TMRM, which is commonly used to assess mitochondrial function by live-cell fluorescence microscopy or flow cytometry. TMRM specifically recognizes and measures mitochondrial membrane potential and is also used to selectively label mitochondria in living cells. The maximum excitation/emission light is 552/575nm[1].TMRM incorporated into cells is preferentially retained in mitochondria, producing bright fluorescence, and the fluorescence intensity is proportional to the mitochondrial membrane potential. Loss of mitochondrial integrity or excessive opening of the mitochondrial permeability transition pore can cause the probe to leak out of the mitochondria, resulting in reduced fluorescence[2]. Rhodamine dye has low toxicity to cells at a certain concentration, so it is often used to detect mitochondria in animal cells, plant cells, and microorganisms[3].
References:
[1]. Michael B Schultz,et. Molecular and Cellular Characterization of SIRT1 Allosteric Activators. 2019:1983:133-149. doi: 10.1007/978-1-4939-9434-2_8.
[2]. Cristiano Ferlini, Giovanni Scambia. Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange. 2007;2(12):3111-4. doi: 10.1038/nprot.2007.397.
[3]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.
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