(±)8(9)-EET MaxSpec® Standard (Synonyms: (±)8,9EpETrE)

(±)8(9)-EET is a fully racemic version of the R/S enantiomeric forms biosynthesized from arachidonic acid . (±)8(9)-EET is a major cytochrome P450 (CYP450) metabolite in the renal cortex. It activates PPARα in HEK293 cells when used at a concentration of 1 µM but inhibits NF-κB activity induced by IL-1β in a PPARα-dependent and -independent fashion. It is also a substrate of COX-1 and COX-2, as measured by oxygen consumption and product formation assays when used at a concentration of 50 µM. (8S,9R)-EET (1 µg/kg), but not (8R,9S)-EET, reduces the glomerular filtration rate (GFR) through COX-dependent pre-glomerular vasoconstriction in rats.(±)8(9)-EET MaxSpec® standard is a quantitative grade standard of (±)8(9)-EET that has been prepared specifically for mass spectrometry or any application where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This (±)8(9)-EET MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stabilitytesting is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.