CRAMP (mouse) |
Catalog No.GA21286
One-Click Copy Product Info
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Le CRAMP (mouse) est un peptide antimicrobien apparentée à la cathelicidine exprimée chez les souris embryonnaires et adultes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 376364-36-2
Sample solution is provided at 25 µL, 10mM.
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Le CRAMP (mouse) est un peptide antimicrobien apparentée à la cathelicidine exprimée chez les souris embryonnaires et adultes[1]. Les AMPs exercent divers effets immunomodulateurs qui peuvent être dominants dans des conditions physiologiques et perdre leurs propriétés bactéricides dans les environnements sériques et tissulaires [2]. Le CRAMP (mouse) est un antibiotique efficace contre les bactéries à Gram négatif, inhibant la croissance de plusieurs souches bactériennes et augmentant directement la perméabilité de la membrane interne d’Escherichia colis [3]. Les souris dépourvues de CRAMP sont plus sensibles aux infections pneumococciques du système nerveux central, entraînant une charge bactérienne plus élevée dans le cerveau et en dehors du système nerveux central, ainsi qu’une infiltration réduite de neutrophiles et une mortalité accrue [4]. Le CRAMP (mouse) est capable d’améliorer la réponse chimiotactique des cellules souches/progénitrices dérivées de la moelle osseuse (BMSPCs) en réponse à un faible gradient de facteurs dérivé du stroma 1 (SDF-1)[5].
References:
[1] Gallo R L, Kim K J, Bernfield M, et al. Identification of CRAMP, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse[J]. Journal of Biological Chemistry, 1997, 272(20): 13088-13093.
[2] Xia X, Cheng L, Zhang S, et al. The role of natural antimicrobial peptides during infection and chronic inflammation[J]. Antonie Van Leeuwenhoek, 2018, 111: 5-26.
[3] Mishra N M, Briers Y, Lamberigts C, et al. Evaluation of the antibacterial and antibiofilm activities of novel CRAMP–vancomycin conjugates with diverse linkers[J]. Organic & Biomolecular Chemistry, 2015, 13(27): 7477-7486.
[4] Kress E, Merres J, Albrecht L J, et al. CRAMP deficiency leads to a pro-inflammatory phenotype and impaired phagocytosis after exposure to bacterial meningitis pathogens[J]. Cell Communication and Signaling, 2017, 15: 1-15.
[5] Klyachkin Y M, Idris A, Rodell C B, et al. Cathelicidin related antimicrobial peptide (CRAMP) enhances bone marrow cell retention and attenuates cardiac dysfunction in a mouse model of myocardial infarction[J]. Stem cell reviews and reports, 2018, 14: 702-714.
| Cas No. | 376364-36-2 | SDF | |
| Formula | C178H302N50O46 | M.Wt | 3878.67 |
| Solubility | DMSO : 3.33 mg/mL (0.86 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 257.8 μL | 1.2891 mL | 2.5782 mL |
| 5 mM | 51.6 μL | 257.8 μL | 515.6 μL |
| 10 mM | 25.8 μL | 128.9 μL | 257.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)