Cyanine5 azide |
Catalog No.GC59117
One-Click Copy Product Info
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Cyanine5 azide est un dérivé du colorant fluorescent. Son usage est fréquent dans les réactions click de type CuAAC.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1267539-32-1 (chloride)
Sample solution is provided at 25 µL, 10mM.
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Cyanine5 azide est un dérivé du colorant fluorescent. Son usage est fréquent dans les réactions click de type CuAAC [1]. Cyanine5 azide peut réagir avec des protéines de type avidine tétravalente monomérique (scAVD) marquées par mCherry pour former une nanoprobe tétrafluorophore. Cette nanoprobe permet le suivi du remodelage du réticulum endoplasmique avec une résolution spatiale nanométrique [2]. Cyanine5 azide est également utilisé pour incuber des cellules afin de détecter d'éventuels groupements DBCO présents à la surface cellulaire. Il permet aussi de fonctionnaliser des nanocomplexes de silice pour un ciblage efficace[3]. Par ailleurs, on peut le conjuguer aux fonctions alcyne d'oligonucléotides phosphoramidate morpholine(PMO) couplés à des peptides. Cette approche permet d'étudier l'absorption cellulaire des complexes peptide-PMO dans des cellules endothéliales de cerveau murin(bEND5)[4].
References:
[1] Huppelschoten Y, Elhebieshy A F, Hameed D S, et al. Total chemical synthesis of a functionalized GFP nanobody[J]. Chembiochem, 2022, 23(19): e202200304.
[2] Hou Y, Arai S, Kitaguchi T, et al. Intracellular bottom-up generation of targeted nanosensors for single-molecule imaging[J]. Nanoscale, 2016, 8(6): 3218-3225.
[3] Wang H, Tang L, Liu Y, et al. In vivo targeting of metabolically labeled cancers with ultra-small silica nanoconjugates[J]. Theranostics, 2016, 6(9): 1467.
[4] Shabanpoor F, Gait M J. Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry[J]. Chemical Communications, 2013, 49(87): 10260-10262.
| Cas No. | 1267539-32-1 (chloride) | SDF | |
| Formula | C35H45ClN6O | M.Wt | 601.22 |
| Solubility | soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6633 mL | 8.3164 mL | 16.6328 mL |
| 5 mM | 332.7 μL | 1.6633 mL | 3.3266 mL |
| 10 mM | 166.3 μL | 831.6 μL | 1.6633 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)