Hyocholic Acid-d4 (Synonyms: HCA-d4, γ-MCA-d4, γ-Muricholic Acid-d4) |
Catalog No.GC48553
One-Click Copy Product Info
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Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Hyocholic acid-d4 is intended for use as an internal standard for the quantification of hyocholic acid by GC- or LC-MS. Hyocholic acid is a primary bile acid in pigs and other mammals.1 It has also been found in urine samples from patients with cholestasis.2 Hyocholic acid is converted by gut microflora primarily to taurohyocholate and, to a lesser extent, taurocholic acid and tauro-β-muricholic acid in mice.3 Hyocholic acid has low toxicity against human hepatoma HepG2 cells.3,4
1.Lundell, K., and Wikvall, K.Species-specific and age-dependent bile acid composition: Aspects on CYP8B and CYP4A subfamilies in bile acid biosynthesisCurr. Drug Metab.9(4)323-331(2008) 2.van Berge Henegouwen, G.P., Brandt, K.H., Eyssen, H., et al.Sulphated and unsulphated bile acids in serum, bile, and urine of patients with cholestasisGut17(11)861-869(1976) 3.Wang, D.Q.-H., Tazuma, S., Cohen, D.E., et al.Feeding natural hydrophilic bile acids inhibits intestinal cholesterol absorption: Studies in the gallstone-susceptible mouseAm. J. Physiol Gastrointest. Liver Physiol.285(3)G494-G502(2003) 4.Perreault, M., Bialek, A., Trottier, J., et al.Role of glucuronidation for hepatic detoxification and urinary elimination of toxic bile acids during biliary obstructionPLoS One8(11)e80994(2013)
| Cas No. | SDF | ||
| Synonymes | HCA-d4, γ-MCA-d4, γ-Muricholic Acid-d4 | ||
| Canonical SMILES | C[C@H](CCC(O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)[C@H](O)[C@]4([H])C([2H])([2H])[C@H](O)C([2H])([2H])C[C@]4(C)[C@@]3([H])CC[C@@]21C | ||
| Formula | C24H36D4O5 | M.Wt | 412.6 |
| Solubility | DMF: 30 mg/ml,DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4237 mL | 12.1183 mL | 24.2365 mL |
| 5 mM | 484.7 μL | 2.4237 mL | 4.8473 mL |
| 10 mM | 242.4 μL | 1.2118 mL | 2.4237 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)