Mito-FerroGreen |
Catalog No.GC20174
One-Click Copy Product Info
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Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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This plan only provides a guide, please modify it to meet your specific needs.
1. Prepare dyeing solution
(1) Prepare stock solution: Add 53μl anhydrous DMSO to a tube containing 50μg Mito-FerroGreen, pipet and mix with a pipette to prepare a Mito-FerroGreen stock solution with a concentration of 1mM.
(2) Prepare working solution: Use serum-free culture medium or HBSS buffer to dilute Mito-FerroGreen stock solution and prepare Mito-FerroGreen working solution with a concentration of 5μM.
Notice:
①Neither the 1 mM Mito-FerroGreen stock solution nor the 5μM Mito-FerroGreen working solution can be stored for a long time, and it is recommended to prepare it for immediate use;
②Since the degradation of Mito-FerroGreen will increase the background fluorescence, if it cannot be used up at one time, it is not recommended to continue to use the remaining Mito-FerroGreen stock solution and working solution;
③Ethanol can be used instead of DMSO. The working solution prepared with ethanol can be stored stably for 2 weeks at -20°C;
④Do not use serum-containing culture media when preparing working solutions. Serum will cause excessive staining background.
2. Cell staining
(1) After inoculating cells on a culture dish/copolymer dish/sterile coverslip, culture in a 37°C, 5% CO2 incubator overnight or until the cells are completely attached;
(2) After removing the medium, wash the cells three times with serum-free medium or HBSS buffer;
(3) Add Mito-FerroGreen working solution with a concentration of 5μM, and incubate for 30 minutes at 37°C and 5% CO2;
(4) Remove the supernatant and wash the cells three times with serum-free medium or HBSS buffer.
(5) (optional, positive control group) Add culture medium containing activators (such as Erastin, RSL3), and culture in a 37°C, 5% CO2 incubator for 1 hour;
Notice:
①Please optimize the specific culture time according to the experimental conditions;
②The order of steps (3) and (5) can be changed according to specific experimental conditions.
3.Observe cells under a fluorescence microscope. The maximum absorption/emission wavelength of Mito-FerroGreen is 505/535nm.
Precautions:
1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.
2) For your safety and health, please wear a lab coat and disposable gloves.
| Cas No. | SDF | ||
| Formula | M.Wt | ||
| Solubility | Soluble in acetonitrile, methanol, dimethanol | Storage | Store at -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)