Biotin-PEG3-azide (Synonyms: Azide-PEG3-biotin) |
Catalog No.GC42939
One-Click Copy Product Info
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Biotin-PEG3-azide es la forma PEGified de biotin-azide y se puede utilizar en la síntesis de PROTACs
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 875770-34-6
Sample solution is provided at 25 µL, 10mM.
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Biotin-PEG3-azide es la forma PEGified de biotin-azide y se puede utilizar en la síntesis de PROTACs [1]. Biotin-PEG3-azide se puede utilizar para etiquetar el alquino termial a través de una reacción de clic catalizada por cobre o una reacción de clic de descobre con ciclooctina [2]. Biotin-PEG3-azide se puede utilizar en reacciones no enzimáticas y marcado covalente de proteínas, y se puede separar y detectar mediante métodos de laboratorio comunes como SDS-PAGE y Western blotting [3].
References:
[1] Flaxman H A. Structural and mechanistic studies of molecular glues and their targets[D]. Harvard University, 2022.
[2] Ge C, Chen X, Wang D. An array of femtoliter wells for sensitive detection of copper using click chemistry[J]. Talanta, 2024, 274: 125973.
[3] Zheng J, Mao Y, Feng S, et al. Combining Metabolic Alkyne Labeling and Click Chemistry for Secretome Analysis of Serum‐Containing Conditioned Medium[J]. Chinese Journal of Chemistry, 2021, 39(7): 1843-1848.
Análisis de microscopía de fluorescencia de sondas intracelulares [1]:
1. Coloque las células en una fuente de cultivo de 35 mm recubierta con poli-L-lisina (con una concentración del 1% en DPBS) y permita que crezcan hasta aproximadamente un 50 % de densidad celular.
2. Lave las células tres veces con DPBS y cultúrelas en un medio que contenga 10 μM de la sonda objetivo (10 μL de solución comercial de 1 mM disuelta en DMSO) durante 4 horas (el tiempo de incubación y la concentración se determinan de acuerdo con las instrucciones proporcionadas por el material de la sonda comprado).
3. Después de la incubación, lave las células tres veces con DPBS y incubelas a temperatura ambiente durante 15 minutos en formalina al 4% (en DPBS).
4. Las células fijas se enjuagan a fondo e incuban a temperatura ambiente durante 1 hora con Click Master Mix (Biotin-PEG3-azide 10μM, THPTA 100mM, ascorbato de sodio 100mM y CuSO4 15mM).
5. Las células se lavan tres veces con DPBS, tres veces con solución de cloruro de sodio anhidra de 500 mM y luego se enjuagan con agua desionizada.
6. Las células se incuban en la oscuridad con estreptavidina-A488 (1 ml, preparada diluyendo la solución madre de 1 mg/mL con DPBS 1:1000) durante 30 minutos. Las células se lavan con DPBS y se incuban a temperatura ambiente con DAPI (1 ml, 50 nM disueltos en DPBS) durante 5 minutos. Finalmente, las células se lavan con DPBS y se someten a imágenes de fluorescencia.
Este protocolo solo proporciona una guía y debe modificarse de acuerdo con sus necesidades específicas.
References:
[1] Kundu S, Jaiswal M, Babu Mullapudi V, et al. Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction[J]. Chemistry–A European Journal, 2024, 30(8): e202303047.
| Cas No. | 875770-34-6 | SDF | |
| Sinónimos | Azide-PEG3-biotin | ||
| Canonical SMILES | O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)([H])N1 | ||
| Formula | C18H32N6O5S | M.Wt | 444.5 |
| Solubility | DMF: 10 mg/ml,DMSO: 10 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2497 mL | 11.2486 mL | 22.4972 mL |
| 5 mM | 449.9 μL | 2.2497 mL | 4.4994 mL |
| 10 mM | 225 μL | 1.1249 mL | 2.2497 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)