TRAP-5 amide (Synonyms: SFLLR-NH2) |
Catalog No.GA23637
One-Click Copy Product Info
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TRAP-5 amide, también conocida como SFLLR-amide, es un péptido agonista activado por proteasa 1 (PAR 1).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 141923-41-3
Sample solution is provided at 25 µL, 10mM.
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TRAP-5 amide, también conocida como SFLLR-amide, es un péptido agonista activado por proteasa 1 (PAR 1) [1]. PAR 1 es el receptor primario sensible a la trombina en las células humanas y juega un papel significativo en las respuestas a la lesión tisular y los procesos inflamatorios relacionados[2]. TRAP-5 amide imita la acción de la trombina y activa el receptor de trombina PAR 1 sin necesidad de proteólisis, lo que la hace útil para estudiar los mecanismo de activación PAR 1[3]. TRAP-5 amide es el péptido más pequeño que puede liberar serotonina de las plaquetas humanas y retener plena actividad, con la secuencia Ser-Phe-Leu-Leu-Arg-NH2[4].
In vitro, el tratamiento de células A549 con amida TRAP-5 amide (10 μM) durante 30 minutos en condiciones estáticas resultó en una redistribución de masa dunámica positiva (DMR) similar a la causada por una dosis saturante de trombina (10 unidades/ml), ay este efecto duró al menos 2 horas. Además, TRAP-5 amide y la trombina mostraron desensibilización cruzada mutua[3].
References:
[1] Ceruso M A, McComsey D F, Leo G C, et al. Thrombin receptor-activating peptides (TRAPs): Investigation of bioactive conformations via structure–activity, spectroscopic, and computational studies[J]. Bioorganic & medicinal chemistry, 1999, 7(11): 2353-2371.
[2] Chen* C, Maryanoff* B E, Andrade-Gordon P. Thrombin receptor modulators: Medicinal chemistry, biological evaluation, and clinical application[J]. Thrombin: Physiology and Disease, 2009: 205-236.
[3] Goral V, Wu Q, Sun H, et al. Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor[J]. FEBS letters, 2011, 585(7): 1054-1060.
[4] Sabo T, Gurwitz D, Motola L, et al. Structure-activity studies of the thrombin receptor activating peptide[J]. Biochemical and biophysical research communications, 1992, 188(2): 604-610.
| Experimentos celulares [1]: | |
Líneas celulares | Células A549 |
Método de preparación | La redistribución dinámica de la masa (DMR) de las células A549 que responden a TRAP-5 amide (10μM) obtenida bajo las condiciones estáticas y de flujo, continua durante 30 min. |
Condiciones de reacción | 10μM; 30 min |
Áreas de aplicación | Bajo la condición de estimulación estática, tanto TRAP-5 amide (10μM) como la trombina (10unidades/ml), cada una a una dosis de saturación, dieron como resultado un DMR positivo similar que se mantiene durante al menos 2 h. |
References: | |
| Cas No. | 141923-41-3 | SDF | |
| Sinónimos | SFLLR-NH2 | ||
| Chemical Name | H-SER-PHE-LEU-LEU-ARG-NH2 | ||
| Formula | C30H51N9O6 | M.Wt | 633.79 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5778 mL | 7.889 mL | 15.7781 mL |
| 5 mM | 315.6 μL | 1.5778 mL | 3.1556 mL |
| 10 mM | 157.8 μL | 788.9 μL | 1.5778 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)