Methylene Blue (hydrate) (Synonyms: NSC 617593, Tetramethylthionine) |
Catalog No.GC47663
One-Click Copy Product Info
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El hidrato de azul de metileno (Basic Blue 9) es un inhibidor de la guanilil ciclasa (sGC), la monoamino oxidasa A (MAO-A) y la NO sintasa (NOS).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 122965-43-9
Sample solution is provided at 25 µL, 10mM.
Methylene blue is a cationic thiazine dye that has diverse biological activities.1,2,3 It inhibits the catalytic function of inducible and constitutive nitric oxide synthases via oxidation of enzyme-bound ferrous iron.1 Methylene blue also inhibits monoamine oxidase (MAO) and increases the activity of mitochondrial cytochrome C oxidase (complex IV).2,3 In vivo, methylene blue stabilizes systemic circulation without increasing peripheral resistance, decreases lipid peroxidation, and reduces anoxic tissue injury in brain and heart in a porcine model of global ischemia and reperfusion injury.1 It inhibits MAO activity in brain and reduces the age-related decline in grip strength and spatial memory in old mice when administered in drinking water at a concentration of 250 μM.2 Methylene blue (15, 30, and 60 mg/kg) also reduces immobility time in the forced swim test in mice, indicating antidepressant-like activity.3 Methylene blue is commonly used to stain cells and tissues in research.4
1.Wiklund, L., Basu, S., Miclescu, A., et al.Neuro- and cardioprotective effects of blockade of nitric oxide action by administration of methylene blueAnn. N.Y. Acad. Sci.1122(1)231-244(2007) 2.Gharib, A., and Atamna, H.Methylene blue induces mitochondrial complex IV and improves cognitive function and grip strength in old miceNeurodegeneration: Theory, disorders and treatments63-85(2011) 3.Harvey, B.H., Duvenhage, I., Viljoen, F., et al.Role of monoamine oxidase, nitric oxide synthase and regional brain monoamines in the antidepressant-like effects of methylene blue and selected structural analoguesBiochem. Pharmacol.80(10)1580-1591(2010) 4.Hu, X., Laguerre, V., Packert, D., et al.A simple and efficient method for preparing cell slides and staining without using cytocentrifuge and cytoclipsInt. J. Cell Biol.2015:813216813216(2015)
| Cas No. | 122965-43-9 | SDF | |
| Sinónimos | NSC 617593, Tetramethylthionine | ||
| Canonical SMILES | CN(C)C1=CC2=[S+]C3=C(C=CC(N(C)C)=C3)N=C2C=C1.[Cl-].O | ||
| Formula | C16H18N3S.Cl [XH2O] | M.Wt | 319.9 |
| Solubility | DMF: 2 mg/ml,DMSO: 2 mg/ml,Ethanol: 3.3 mg/ml,PBS (pH 7.2): 0.14 mg/ml | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.126 mL | 15.6299 mL | 31.2598 mL |
| 5 mM | 625.2 μL | 3.126 mL | 6.252 mL |
| 10 mM | 312.6 μL | 1.563 mL | 3.126 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >96.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 32 reference(s) in Google Scholar.)