(±)12-HETE-d8 (Synonyms: (±)12-Hydroxyeicosatetraenoic Acid-d8) |
| Catalog No.GC48738 |
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2525175-25-9
Sample solution is provided at 25 µL, 10mM.
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(±)12-HETE-d8 is intended for use as an internal standard for the quantification of 12-HETE by GC- or LC-MS. (±)12-HETE is formed via non-enzymatic oxidation of arachidonic acid .1,2 12(S)- and 12(R)-HETE are formed by 12(S)- and 12(R)-lipoxygenase-mediated oxidation of arachidonic acid, respectively.3,4 12(R)-HETE can also be formed by oxidation of arachidonic acid mediated by cytochrome P450s (CYP450s).5
1.Powell, W.S., and Rokach, J.Biosynthesis, biological effects, and receptors of hydroxyeicosatetraenoic acids (HETEs) and oxoeicosatetraenoic acids (oxo-ETEs) derived from arachidonic acidBiochim. Biophys. Acta1851(4)340-355(2014) 2.Derogis, P.B.M.C., Chaves-Filho, A.B., and Miyamoto, S.Characterization of hydroxy and hydroperoxy polyunsaturated fatty acids by mass spectrometryBioactive lipids in health and disease21-38(2019) 3.KÜhn, H., Banthiya, S., and van Leyen, K.Mammalian lipoxygenases and their biological relevanceBiochim. Biophys. Acta1851(4)308-330(2015) 4.BÜrger, F., Krieg, P., Marks, F., et al.Positional- and stereo-selectivity of fatty acid oxygenation catalysed by mouse (12S)-lipoxygenase isoenzymesBiochem J.348(Pt 2)329-335(2000) 5.Powell, W.S., and Rokach, J.Biosynthesis, biological effects, and receptors of hydroxyeicosatetraenoic acids (HETEs) and oxoeicosatetraenoic acids (oxo-ETEs) derived from arachidonic acidBiochim. Biophys. Acta1851(4)340-355(2015)
| Cas No. | 2525175-25-9 | SDF | |
| Synonymes | (±)12-Hydroxyeicosatetraenoic Acid-d8 | ||
| Canonical SMILES | CCCCC/C([2H])=C([2H])\CC(O)([2H])/C([2H])=C/C([2H])=C([2H])\C/C([2H])=C([2H])\CCCC(O)=O | ||
| Formula | C20H24D8O3 | M.Wt | 328.5 |
| Solubility | 0.1 M Na2CO3: 2 mg/ml,DMF: miscible,DMSO: miscible,Ethanol: miscible,PBS (pH 7.4): 0.8 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0441 mL | 15.2207 mL | 30.4414 mL |
| 5 mM | 0.6088 mL | 3.0441 mL | 6.0883 mL |
| 10 mM | 0.3044 mL | 1.5221 mL | 3.0441 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)
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