Arachidonoyl p-Nitroaniline (Synonyms: ApNA) |
| Catalog No.GC42842 |
Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.
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Cas No.: 119520-58-0
Sample solution is provided at 25 µL, 10mM.
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Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity. FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide . It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.
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