Brassinazole |
| Catalog No.GC19544 |
Le brassinazole, composé de type triazole, est un inhibiteur de la biosynthèse des brassinostéroÏdes (BR).
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Cas No.: 224047-41-0
Sample solution is provided at 25 µL, 10mM.
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Brassinazole (Brz) is a specific inhibitor of C-22 hydroxylase (CYP90B1) in brassinosteroid (BR) biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs[1]. Brassinazole has regulatory roles in G-fiber cell wall development and secondary xylem cell wall carbohydrate biosynthesis during tension wood formation in hybrid poplar[2]. In addition, brassinazole treatment (5µM) can delay persimmon fruit ripening and suppress respiration rate[3].
Brassinazole treatment (5, 1, and 0.5μM) markedly caused dwarfism of Arabidopsis seedlings in a concentration-dependent manner. Moreover, brassinazole treatment (1μM) showed a BR-deficient mutant-like phenotype, which is rescued by the application of brassinolide (BL) (10nM). Dark-grown 10-d-old Arabidopsis thaliana seedlings were treated with different concentrations of brassinozoles from 0 to 10μM . As the concentration of brassinozole increased, hypocotyl length decreased. The change in length occurred between 0.1 and 0.5μM[4].
References:
[1]. Oh K, Matsumoto T, Yamagami A, Ogawa A, Yamada K, Suzuki R, et al. YCZ-18 is a new brassinosteroid biosynthesis inhibitor. PLoS One. 2015;10(3):e0120812. Epub 20150320. doi: 10.1371/journal.pone.0120812. PubMed PMID: 25793645.
[2]. Gao J, Yu M, Zhu S, Zhou L, Liu S. Effects of exogenous 24-epibrassinolide and brassinazole on negative gravitropism and tension wood formation in hybrid poplar (Populus deltoids X Populus nigra). Planta. 2019;249(5):1449-63. Epub 20190128. doi: 10.1007/s00425-018-03074-2. PubMed PMID: 30689054.
[3]. He Y, Li J, Ban Q, Han S, Rao J. Role of Brassinosteroids in Persimmon ( Diospyros kaki L.) Fruit Ripening. J Agric Food Chem. 2018;66(11):2637-44. Epub 20180312. doi: 10.1021/acs.jafc.7b06117. PubMed PMID: 29509414.
[4]. Asami T, Min YK, Nagata N, Yamagishi K, Takatsuto S, Fujioka S, et al. Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor. Plant Physiol. 2000;123(1):93-100. doi: 10.1104/pp.123.1.93. PubMed PMID.
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