Calcein Blue |
| Catalog No.GC43117 |
Calcein Blue, un colorant fluorescent imperméable À la membrane, est un dérivé de la coumarine qui contient une structure d'acide iminodiacétique.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 54375-47-2
Sample solution is provided at 25 µL, 10mM.
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Calcein Blue is a short-term, blue-fluorescent dye.
Calcein blue AM is only weakly fluorescent (excitation/emission maxima 322/435 nm). Upon cleavage of the AM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for several hours. In addition, its fluorescence intensity increases and its fluorescence spectra shift to longer wavelengths, with excitation/emission maxima of 360/449 nm. The fluorescence of Calcein Blue is known to be quenched in the presence of iron(III); if a stronger chelator removes this ion from the fluorophore, the fluorescence of the fluorophore is regained[1]. A novel fluorimetric assay for dopamine using calcein blue (CB) complexed with Fe2 ion as a chemical sensor is described. The fluorescence arising from calcein blue of the calcein blue–Fe2 complex is quenched by the Fe2 ion. When dopamine is added to a solution of the calcein blue–Fe2 complex, a dopamine–Fe2 complex is formed as the result of a ligand exchange reaction between calcein blue and dopamine which permits the fluorescence from calcein blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the calcein blue–Fe2 complex solution, which permits dopamine to be quantitatively determined[2].
References:
[1]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.
[2]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43.
Cell experiment: | The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader[1]. |
References: [1]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49. | |
| Cas No. | 54375-47-2 | SDF | |
| Canonical SMILES | OC1=CC=C(C(C)=CC(O2)=O)C2=C1CN(CC(O)=O)CC(O)=O | ||
| Formula | C15H15NO7 | M.Wt | 321.3 |
| Solubility | DMSO : 1 mg/mL (3.11 mM; Need ultrasonic); H2O : < 0.1 mg/mL (insoluble) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.1124 mL | 15.5618 mL | 31.1236 mL |
| 5 mM | 622.5 μL | 3.1124 mL | 6.2247 mL |
| 10 mM | 311.2 μL | 1.5562 mL | 3.1124 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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