CTB (Synonyms: Cholera Toxin B subunit) |
Catalog No.GC19891
One-Click Copy Product Info
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Le CTB est un puissant activateur de l'histone acétyltransférase p300.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 451491-47-7
Sample solution is provided at 25 µL, 10mM.
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CTB (Cholera Toxin B subunit) is a potent p300 histone acetyltransferase activator[1]. CTB can effectively induce apoptosis in MCF-7 cells[2].
CTB (10, 50, 100, 150, 200, and 250 µM; 10 min) enhances p300 HAT activity of p300 in a dosedependent manner[1].
CTB (0-200 μM; 24 hours) inhibits the viability of MCF-7 cells with an IC50 of 85.43 μM[2].
CTB (85.43 μM; 24, 48 and 72 h) induces time dependence apoptosis of MCF-7 cells[2].
CTB (50 uM; 24 h) increases p300/CBP activity , and reduces autophagic flux in primary neurons[3].
Reference:
[1]. Mantelingu K, et al. Activation of p300 histone acetyltransferase by small molecules altering enzyme structure: probed by surface-enhanced Raman spectroscopy. J Phys Chem B. 2007;111(17):4527-4534.
[2]. Dastjerdi MN, et al. The effect of CTB on P53 protein acetylation and consequence apoptosis on MCF-7 and MRC-5 cell lines. Adv Biomed Res. 2013;2:24. Published 2013 Mar 6.
[3]. Chen X, et al. Promoting tau secretion and propagation by hyperactive p300/CBP via autophagy-lysosomal pathway in tauopathy. Mol Neurodegener. 2020;15(1):2. Published 2020 Jan 6.
| Cas No. | 451491-47-7 | SDF | |
| Synonymes | Cholera Toxin B subunit | ||
| Chemical Name | Cholera Toxin B subunit | ||
| Formula | C₁₆H₁₃ClF₃NO₂ | M.Wt | 343.73 |
| Solubility | ethanol: 5 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9093 mL | 14.5463 mL | 29.0926 mL |
| 5 mM | 581.9 μL | 2.9093 mL | 5.8185 mL |
| 10 mM | 290.9 μL | 1.4546 mL | 2.9093 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)