DiZPK Hydrochloride |
Catalog No.GC19125 |
Le chlorhydrate de DiZPK en tant que sonde présente deux caractéristiques, la première étant que le chlorhydrate de DiZPK en tant que dérivé de la pyrrolysine peut être utilisé pour la technologie d'expansion du codon génétique.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
DiZPK Hydrochloride as a probe has two characteristics, the first DiZPK Hydrochloride as a derivative of pyrrolysine (the 22nd natural amino acid) can be used for genetic codon expansion technology; The non-natural amino acid can be introduced into the stop codon TAG position through the modification of pyrrole lyricyl-TrNA synthase (PylRS) and its corresponding tRNA(tRNAPyl) system. In addition, DiZPK Hydrochloride contains diazirine photocrosslinking group at specific 365 nm. Under wavelength irradiation, bisacproridine can be converted to free radical group, thus realizing the transient protein-protein non-covalent conversion to covalent action. Compared with other photocrosslinked groups, the "short" structure of the Linker with bisacridine can minimize the impact on the function of the target protein[1].
DiZPK Hydrochloride revealed a crosslinking efficiency of 60% within 1 min of UV exposure and achieved a maximum efficiency higher than 80% within 3 min demonstrating the high flexibility of DiZPK[2].
By site-specific introduction of DiZPK photoaffinity probes onto HdeA, it combines with gel-based proteomics to allow substrate analysis of HdeA in living E.coli cells at very low pH. Two important periplasmic partners, DegP and SurA, were found in the natural substrates identified by mass spectrometry, initially protected by HdeA at low pH, but subsequently assisting in HDEa-mediated refolding of other proteins after neutralization[2].
References:
[1]. He D, Xie X, et,al. Quantitative and Comparative Profiling of Protease Substrates through a Genetically Encoded Multifunctional Photocrosslinker. Angew Chem Int Ed Engl. 2017 Nov 13;56(46):14521-14525. doi: 10.1002/anie.201708151. Epub 2017 Oct 11. PMID: 28940571.
[2]. Zhang M, Lin S, et,al. A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance. Nat Chem Biol. 2011 Sep 4;7(10):671-7. doi: 10.1038/nchembio.644. PMID: 21892184.
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