Thidiazuron (Synonyms: CCG-24904, SN 49537, TDZ) |
Catalog No.GC45032
One-Click Copy Product Info
|
Thidiazuron fait partie des dérivés d'urée disubstitués. Il possède une très forte activité cytokinine, on l'utilise beaucoup comme herbicide et régulateur de croissance végétale.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 51707-55-2
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Thidiazuron fait partie des dérivés d'urée disubstitués. Il possède une très forte activité cytokinine, on l'utilise beaucoup comme herbicide et régulateur de croissance végétale[1]. Thidiazuron agit sur plusieurs mécanismes de morphogenèse chez les plantes : on peut notamment citer l'apparition des tiges axillaires, la régénération de tiges adventices et l'embryogenèse somatique, sur un grand nombre d'espèces végétales[2].
Thidiazuron (0~50μM; 24h) réduit de manière dose-dépendante l'expression de uPA, uPAR, MMPs et VEGF dans les cellules MDA-MB-231[3]. Thidiazuron (0, 0.025, 0.05, 0.1 et 0.4mg/L; 4 semaines) augmente l'expression de la cytokinine oxydase[2]. Thidiazuron (0, 2.3, 4.5, 6.9 et 9μM; 10 semaines) stimule la formation d'embryons somatiques dans les explants de fraisier[4].
References:
[1] Nisler J. In Thidiazuron: From Urea Derivative to Plant Growth Regulator [M]. Berlin/Heidelberg, Germany: Springer, 2018.
[2] Wang F, Li Y, Pang Y, et al. Thidiazuron enhances strawberry shoot multiplication by regulating hormone signal transduction pathways [J]. International journal of molecular sciences, 2025, 26(9): 4060.
[3] Rajendran P, Ben Ammar R, Al-Saeedi F J, et al. Thidiazuron decreases epithelial-mesenchymal transition activity through the NF-kB and PI3K/AKT signalling pathways in breast cancer [J]. Journal of cellular and molecular medicine, 2020, 24(24): 14525-14538.
[4] Ghosh A, Igamberdiev A U, Debnath S C. Thidiazuron-induced somatic embryogenesis and changes of antioxidant properties in tissue cultures of half-high blueberry plants [J]. Scientific reports, 2018, 8(1): 16978.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules MDA-MB-231 |
Méthode de préparation | Les cellules ont été cultivées sur les lames à quatre chambres, exposées à Thidiazuron (50μM) pendant 2h, fixées et perméabilisées. Les cellules ont été incubées avec un anticorps anti-p65, puis avec un anticorps secondaire marqué à FITC. La localisastion subcellulaire de la protéine p65 a été observée au microscope confocal à un grossissement de 40 fois. |
Conditions de réaction | 50μM; 2h |
Domaines d'application | Thidiazuron a inhibé la translocation nucléaire de p65. |
References: | |
| Cas No. | 51707-55-2 | SDF | |
| Synonymes | CCG-24904, SN 49537, TDZ | ||
| Canonical SMILES | O=C(NC1=CN=NS1)NC2=CC=CC=C2 | ||
| Formula | C9H8N4OS | M.Wt | 220.2 |
| Solubility | DMF: 30 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 15 mg/ml,Ethanol: 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.5413 mL | 22.7066 mL | 45.4133 mL |
| 5 mM | 908.3 μL | 4.5413 mL | 9.0827 mL |
| 10 mM | 454.1 μL | 2.2707 mL | 4.5413 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 34 reference(s) in Google Scholar.)