WST-1 (Synonyms: Water-Soluble Tetrazolium 1) |
Catalog No.GC48398
One-Click Copy Product Info
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WST-1 is a water-soluble tetrazolium salt reagent for cell proliferation/cytotoxicity detection.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 150849-52-8
Sample solution is provided at 25 µL, 10mM.
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WST-1 is a water-soluble tetrazolium salt reagent for cell proliferation/cytotoxicity detection. WST-1 can be reduced by dehydrogenase in mitochondria of viable cells to generate water-soluble formazan dye to quantify cellular metabolic activity, and rapidly evaluate cell proliferation, viability and drug toxicity by colorimetry. WST-1 can be used in anti-tumor drug screening, environmental toxicology evaluation and other related research[1-4].
References:[1] Nissen E, Pauli G, Vollenbroich D. WST-1 assay--a simple colorimetric method for virus titration. In Vitro Cell Dev Biol Anim. 1997 Jan;33(1):28-9.
[2] Wiertelak-Makała K, Szymczak-Pajor I, Bociong K, et al. Considerations about Cytotoxicity of Resin-Based Composite Dental Materials: A Systematic Review. Int J Mol Sci. 2023 Dec 21;25(1):152.
[3] Berridge MV, Tan AS. High-capacity redox control at the plasma membrane of mammalian cells: trans-membrane, cell surface, and serum NADH-oxidases. Antioxid Redox Signal. 2000 Summer;2(2):231-42.
[4] Shin SP, Sohn HC, Jin CN, et al. Evaluation of the effect of protease inhibitors on the viability of Miamiensis avidus using the WST-1 assay. Parasitol Int. 2020 Jun;76:102093.
WST-1 assay for cell proliferation (cytotoxicity)[1]
This protocol is compiled based on the research content and for reference only. Please adjust according to actual needs.
1. Reagent and cell preparation
(1) Preparation of WST-1 working solution: dissolve WST-1 in DMSO to prepare 10mg/mL stock solution. Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare WST-1 working solution.
(2) Cell seeding: seed cells in a 96-well culture plate. Add approximately 4×10⁴ viable cells per well. Incubate overnight at 37°C, 5%CO2.
(3) Medium replacement: remove the original medium. Add 100μL phenol red-free serum-free DMEM (DMEM-PRF) per well.
2. WST-1 staining and detection
(1) WST-1 staining: add WST-1 working solution per well (final concentration of 5-10μM; DMSO concentration below 1%). Incubate at room temperature for 2h.
(2) Determination: After incubation, measure the absorbance of each well at 450/690nm using a microplate reader.
3. Data analysis
(1) Calculate the percentage of cytotoxicity:
Cytotoxicity (%)=[(Aexperimental value–Anegative control)/(Apositive control–Anegative control)]×100 (Note: A is the absorbance value).
Precautions:
(1) This method detects metabolic activity. WST-1 reacts with mitochondrial succinate-tetrazolium reductase to produce water-soluble formazan. No additional solubilization step is required.
(2) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:[1] Ngamwongsatit P, Banada PP, Panbangred W, et al. WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J Microbiol Methods. 2008 Jun;73(3):211-5.
| Cas No. | 150849-52-8 | SDF | |
| Synonymes | Water-Soluble Tetrazolium 1 | ||
| Canonical SMILES | [O-]S(C(C=C1)=CC(S([O-])(=O)=O)=C1C2=NN(C3=CC=C(I)C=C3)[N+](C4=CC=C([N+]([O-])=O)C=C4)=N2)(=O)=O.[Na+] | ||
| Formula | C19H11IN5O8S2•Na | M.Wt | 651.3 |
| Solubility | DMF: 5 mg/ml,DMSO: 10 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5354 mL | 7.677 mL | 15.3539 mL |
| 5 mM | 307.1 μL | 1.5354 mL | 3.0708 mL |
| 10 mM | 153.5 μL | 767.7 μL | 1.5354 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)