Apyrase |
Catalog No.GC26593
One-Click Copy Product Info
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Apyrase es una nucleoside triphosphate diphosphohydrolase (NTPDase) que elimina catalíticamente el fosfato terminal de los NTPs y NDPs
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9000-95-7
Sample solution is provided at 25 µL, 10mM.
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Apyrase es una nucleoside triphosphate diphosphohydrolase (NTPDase) que elimina catalíticamente el fosfato terminal de los NTPs y NDPs [1]. La actividad de la Apyrase vegetal se ha estimulado de forma notable por la calmodulina activada por Ca2+[1]. Una unidad de Apyrase se define como la cantidad de enzima que libera 1.0μmol de fosfato inorgánico por minuto a partir de ATP o ADP, a pH 6.5 y 30°C.
Apyrase (20U/mL; 6h) redujo los niveles de RecA en Escherichia coli O157:H7; A concentración de 10 y 20U/mL (6h) disminuyó la cantidad de DNA del fago Stx2 en sobrenadantes de esta bacteria, de manera dependiente de la dosis. A 10U/mL (6h), redujo la libreación de la toxina Shiga 2 por E. coli O157:H7 in vitro [2].
Apyrase (15U; 0, 2d; i.p.) dimó la aparición de síntomas en ratones BALB/c infectados con E. coli O157: H7. Asimismo, redujo la apoptosis de células intestinales y los niveles de ATP en heces de estos animales[2]. Apyrase (2U; i.p.) disminuyó de forma el tamaño tumoral en ratasWistar con glioma implantado [3]. Apyrase (4U/g; i.v.) redujo el número de neutrófilos y macrófagos en el cerebro isquémico de ratones Cd39 knock-out, así como el tamaño del infarto cerebral[4]. Apyrase (0.2U; top) disminuyó los niveles de ATP en la piel de ratones C57BL/6 después de una quemadura. Administrada durante 2 días, inhibió la reproducción bacteriana en las zonas quemadas[5].
References:
[1] Clark G, Brown K A, Tripathy M K, et al. Recent advances clarifying the structure and function of plant apyrases (nucleoside triphosphate diphosphohydrolases) [J]. International journal of molecular sciences, 2021, 22(6): 3283.
[2] Arvidsson I, Tontanahal A, Johansson K, et al. Apyrase decreases phage induction and Shiga toxin release from E. coli O157:H7 and has a protective effect during infection [J]. Gut Microbes, 2022, 14(1): 2122667.
[3] Morrone F B, Oliveira D L, Gamermann P, et al. In vivo glioblastoma growth is reduced by apyrase activity in a rat glioma model [J]. BMC Cancer, 2006, 6: 226.
[4] Hyman M C, Petrovic-Djergovic D, Visovatti S H, et al. Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39 [J]. The Journal of clinical investigation, 2009, 119(5): 1136-1149.
[5] Bayliss J M, Levi B, Wu J, et al. Apyrase elicits host antimicrobial responses and resolves infection in burns [J]. Journal of burn care & research : official publication of the American Burn Association, 2016, 37(6): e501-e507.
| Experimentos celulares [1]: | |
Líneas celulares | Escherichia coli O157:H7 |
Método de preparación | E. coli O157:H7 se incubó con o sin apyrase durante 6h y se analizaron lisados bacterianos para detectar el nivel de RecA. |
Condiciones de reacción | 20U/mL; 6h |
Áreas de aplicación | Apyrase disminuyó los niveles de RecA en Escherichia coli O157:H7. |
| Experimentos con animales [2]: | |
Modelos animales | Ratones hembra C57BL/6 |
Método de preparación | La piel local de los ratones se sumergió en agua destilada a 60°C para producir una quemadura de escaldadura de espesor parcial y luego se infectó con Acinetabacter baumannii resistente a los antibióticos inmediatamente. Un tratamiento diario de 200 μL (1U/mL) de apyrase o vehículo solo se aplicó a la herida de quemadura. Después de 2 días, se cosechó la piel para cuantificar las CFUs. |
Forma de dosificación | 0.2U; 2d; top |
Áreas de aplicación | Apyrase inhibió la reproducción bacteriana en las quemaduras de ratones C57BL/6. |
References: | |
| Cas No. | 9000-95-7 | SDF | |
| Formula | M.Wt | ||
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 217u/mg protein Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)