Apyrase |
Katalog-Nr.GC26593
One-Click Copy Product Info
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Apyrase is a kind of nucleoside triphosphate diphosphohydrolase (NTPDase) that catalytically removes the terminal phosphate from NTPs and NDPs.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9000-95-7
Sample solution is provided at 25 µL, 10mM.
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Apyrase is a kind of nucleoside triphosphate diphosphohydrolase (NTPDase) that catalytically removes the terminal phosphate from NTPs and NDPs [1]. The activity of plant Apyrase is significantly stimulated by Ca2+-activated calmodulin [1]. One unit is defined as the amount of Apyrase that can release 1.0μmol of inorganic phosphate from ATP or ADP per minute at pH 6.5 and 30°C.
Apyrase (20U/mL; 6h) decreased the levels of RecA in Escherichia coli O157:H7; Apyrase (10, 20U/mL; 6h) decreased the amount of Stx2 phage DNA in E. coli O157:H7 supernatants in a dose-dependent manner; Apyrase (10U/mL; 6h) decreased Shiga toxin 2 release from E. coli O157:H7 in vitro [2].
Apyrase (15U; 0, 2d; i.p.) delayed the development of symptoms in BALB/c mice infected with E. coli O157: H7; Apyrase (15U; i.p.) decreased the apoptosis of intestinal cells of the BALB/c mice infected with E. coli O157:H7; Apyrase (15U; i.p.) decreased the ATP in feces from E. coli O157:H7-infected BALB/c mice [2]. Apyrase (2U; i.p.) significantly reduced the size of tumor in the Wistar rats with implanted glioma [3]. Apyrase (4U/g; i.v.) decreased the number of neutrophils and macrophages in the ischemic brain of Cd39-null mice and diminished the size of cerebral infarct[4]. Apyrase (0.2U; top) reduced ATP on skin of C57BL/6 mice after burn; Apyrase (0.2U; 2d; top) inhibited bacterial reproduction in the burns of C57BL/6 mice [5].
References:
[1] Clark G, Brown K A, Tripathy M K, et al. Recent advances clarifying the structure and function of plant apyrases (nucleoside triphosphate diphosphohydrolases) [J]. International journal of molecular sciences, 2021, 22(6): 3283.
[2] Arvidsson I, Tontanahal A, Johansson K, et al. Apyrase decreases phage induction and Shiga toxin release from E. coli O157:H7 and has a protective effect during infection [J]. Gut Microbes, 2022, 14(1): 2122667.
[3] Morrone F B, Oliveira D L, Gamermann P, et al. In vivo glioblastoma growth is reduced by apyrase activity in a rat glioma model [J]. BMC Cancer, 2006, 6: 226.
[4] Hyman M C, Petrovic-Djergovic D, Visovatti S H, et al. Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39 [J]. The Journal of clinical investigation, 2009, 119(5): 1136-1149.
[5] Bayliss J M, Levi B, Wu J, et al. Apyrase elicits host antimicrobial responses and resolves infection in burns [J]. Journal of burn care & research : official publication of the American Burn Association, 2016, 37(6): e501-e507.
| Cell experiment [1]: | |
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Cell lines |
Escherichia coli O157:H7 |
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Preparation Method |
E. coli O157:H7 was incubated with or without apyrase for 6h and bacterial lysates were assayed to detect the level of RecA. |
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Reaction Conditions |
20U/mL; 6h |
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Applications |
Apyrase decreased the levels of RecA in Escherichia coli O157:H7. |
| Animal experiment [2]: | |
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Animal models |
Female C57BL/6 mice |
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Preparation Method |
The local skin of mice was immersed in 60°C distilled water to produce a partial-thickness scald burn and then were infected with antibiotic-resistant Acinetabacter baumannii immediately. A daily treatment of 200 μL (1U/mL) of either apyrase or vehicle alone was applied topically to the burn wound. After 2d, skin was harvested to quantify CFUs. |
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Dosage form |
0.2U; 2d; top |
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Applications |
Apyrase inhibited bacterial reproduction in the burns of C57BL/6 mice. |
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References: |
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| Cas No. | 9000-95-7 | SDF | |
| Formula | M.Wt | ||
| Löslichkeit | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 217u/mg protein Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)