IPTG (Isopropyl β-D-thiogalactoside) (Synonyms: Isopropyl βthiogalactoside) |
Catalog No.GC30002 |
IPTG (Isopropil β-D-tiogalactÓsido) es un mimético molecular de la alolactosa, un metabolito de la lactosa que desencadena la transcripciÓn del operÓn lac y, por lo tanto, se utiliza para inducir la expresiÓn de proteÍnas donde el gen estÁ bajo el control del operador lac.
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Cas No.: 367-93-1
Sample solution is provided at 25 µL, 10mM.
IPTG (isopropyl-β-D-thiogalactopyranoside) is a commonly used inducer used to induce the expression of lactose operon (Lac) downstream genes and corresponding proteins in Escherichia coli [1]. When using pET, pLac or other expression systems controlled by Lac operators, IPTG as an inducer can effectively initiate large-scale expression of target genes [2]. The mechanism of action of IPTG is that its structure is similar to lactose and can bind to the lactose operon repressor protein (LacI), thereby preventing LacI from binding to Lac [3]. IPTG is not a harmless inducer, it exacerbates the toxicity of haloalkane substrates and causes significant damage to host cells [4].
References:
[1] Hansen L H , Knudsen S , Rensen S J .The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens[J].Current Microbiology, 1998, 36(6):341-7.
[2] Saïda F, Uzan M, Odaert B, et al. Expression of highly toxic genes in E. coli: special strategies and genetic tools[J]. Current Protein and Peptide Science, 2006, 7(1): 47-56.
[3] Marbach A , Bettenbrock K. lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA[J].Journal of Biotechnology, 2012, 157(1):82-88.
[4]Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway[J].Microbial Cell Factories, 2015, 14(1):201.
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