N6-Methyladenosine-5'-Triphosphate |
Catalog No.GN20007
One-Click Copy Product Info
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N6-Methyladenosine-5-Triphosphate (N6-metil ATP; m6ATP) es un análogo de adenosina modificado en la base, identificado como un nucleósido menor en el ARN natural.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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N6-Methyladenosine-5-Triphosphate (N6-metil ATP; m6ATP) es un análogo de adenosina modificado en la base, identificado como un nucleósido menor en el ARN natural[1]. La N6-Metiladenosina-5’-Trifosfato es una modificación covalente dinámica, reversible, de ribonucleótidos, comúnmente encontrada en el ARN mensajero (ARNm) eucariota, y desempeña un papel crucial en la regulación del empalme, exportación, estabilidad y traducción del ARN[2, 3]. La N6-Metiladenosina-5-Trifosfato actúa como sustrato para la ARN polimerasa y se utiliza como sustituto completo del ATP en las reacciones de modificación del ARNm[4, 5]. Además, la N6-Metiladenosina-5-Trifosfato es un agonista efectivo para los receptores purinérgicos P2Y en cobayas y en Escherichia coli[6]. Este producto se suministra en forma de una solución de sal de litio.
References:
[1] Yuan Y, Arneson R, Burke E, et al. Efficient 3′-end tailing of RNA with modified adenosine for nanopore direct total RNA sequencing[J]. bioRxiv, 2024: 2024.02. 24.581884.
[2] Zheng H, Li S, Zhang X, et al. Functional implications of active N6-methyladenosine in plants[J]. Frontiers in Cell and Developmental Biology, 2020, 8: 291.
[3] Anderson S J, Kramer M C, Gosai S J, et al. N6-methyladenosine inhibits local ribonucleolytic cleavage to stabilize mRNAs in Arabidopsis[J]. Cell reports, 2018, 25(5): 1146-1157. e3.
[4] Parr C J C, Wada S, Kotake K, et al. N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells[J]. Nucleic Acids Research, 2020, 48(6): e35-e35.
[5] Potapov V, Fu X, Dai N, et al. Base modifications affecting RNA polymerase and reverse transcriptase fidelity[J]. Nucleic Acids Research, 2018, 46(11): 5753-5763.
[6] Burnstock G, Fischer B, Hoyle C H V, et al. Structure activity relationships for derivatives of adenosine‐5′‐triphosphate as agonists at P2 purinoceptors: Heterogeneity within P2x and P2y subtypes[J]. Drug development research, 1994, 31(3): 206-219.
| purity | >99.00% | Extinction Coefficient | 15,567 Lmol-1cm-1 at 265 nm |
| Formula | C11H18N5O13P3 (free acid) | M.Wt | 521.20 g/mole (free acid) |
| Salt Form | Li+ | Concentration | 100 mM |
| Buffer | H2O | Storage | -20°C or below |
| Sinónimos | N6-Methyl-ATP, m6ATP | Backbone | 5'-Triphosphate |
| Base Analog | Adenosine | Sugar Type | RNA |
| Nucleotide Category | Base Modified RNA | ||
| Applications | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies | ||
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)