MPP dihydrochloride |
| Catalog No.GC11881 |
Antagonista silencioso de los receptores ERα
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 911295-24-4
Sample solution is provided at 25 µL, 10mM.
ERα receptors silent antagonist
| Cell experiment [1]: | |
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Cell lines |
Primary hippocampal cells |
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Preparation method |
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
1 pM ~ 1 μM |
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Applications |
In primary hippocampal cells, E4 alone or in combination with MPP Dihydrochloride significantly decreased H2O2-induced LDH activity. Moreover, E4, E4 ﹢MPP Dihydrochloride or E4 ﹢MPP Dihydrochloride ﹢ PHTTP significantly increased the cell survival rate, which suggested that neither inhibition of ERα alone nor inhibition of both receptors (i.e. ERα and ERβ) blocked the E4-dependent cell survival activity. However, E4 ﹢ PHTTP (inhibition of ERβ alone) significantly down-regulated the cell survival rate. |
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References: [1]. Tskitishvili E, Pequeux C, Munaut C, Viellevoye R, Nisolle M, Nol A, Foidart JM. Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study. J Endocrinol. 2017 Jan;232(1):85-95. |
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| Cas No. | 911295-24-4 | SDF | |
| Chemical Name | 4-(1-(4-hydroxyphenyl)-4-methyl-5-(4-(2-(piperidin-1-yl)ethoxy)phenyl)-1H-pyrazol-3(2H)-ylidene)cyclohexa-2,5-dienone dihydrochloride | ||
| Canonical SMILES | OC1=CC=C(C=C1)N(C(C(C=C2)=CC=C2OCCN3CCCCC3)=C/4C)NC4=C(C=C5)\C=CC5=O.Cl.Cl | ||
| Formula | C29H31N3O3.2HCl | M.Wt | 542.5 |
| Solubility | <54.25mg/ml in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8433 mL | 9.2166 mL | 18.4332 mL |
| 5 mM | 0.3687 mL | 1.8433 mL | 3.6866 mL |
| 10 mM | 0.1843 mL | 0.9217 mL | 1.8433 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)
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