Phalloidin (Synonyms: NSC 523214) |
Catalog No.GC15692
One-Click Copy Product Info
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Phalloidin es una toxina derivada de hongos que se puede conjugar a tintes fluorescentes para etiquetar la cytoskeletal F-actin
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17466-45-4
Sample solution is provided at 25 µL, 10mM.
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Phalloidin es una toxina derivada de hongos que se puede conjugar a tintes fluorescentes para etiquetar la cytoskeletal F-actin[1]. Phalloidin es un péptido bicíclico altamente selectivo que funciona uniendo y estabiliando la F-actin, evitando efectivamente la despolimerización del filamento de actina [2]. Los derivados de la Phalloidin que contienen etiquetas fluorescentes se utilizan ampliamente para visualizar la F-actin en la investigación biomédica[3]. Phalloidin interfiere con la motilidad y el crecimiento celular [4]. Phalloidin no es adecuada para células fijadas con metanol, probablemente debido a la interrupción de la integridad del filamento de actina, pero es adecuada para células fijadas en glutaraldehído al 0.2% en PBS[5]. Para RITC-Phalloidin, la longitud de onda máxima de excitación es de 495nm (o 550nm) y la longitud de onda máxima de emisión es de 520nm[5].
References:
[1] DesMarais V, Eddy R J, Sharma V P, et al. Optimizing leading edge F-actin labeling using multiple actin probes, fixation methods and imaging modalities[J]. BioTechniques, 2019, 66(3): 113-119.
[2] Gübeli R J, Bertoldo D, Shimada K, et al. In Vitro-Evolved Peptides Bind Monomeric Actin and Mimic Actin-Binding Protein Thymosin-β4[J]. ACS Chemical Biology, 2021, 16(5): 820-828.
[3] Romani M, Auwerx J. Phalloidin staining of actin filaments for visualization of muscle fibers in Caenorhabditis elegans[J]. Bio-protocol, 2021, 11(19): e4183-e4183.
[4] Wehland J, Osborn M, Weber K. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth[J]. Proceedings of the National Academy of Sciences, 1977, 74(12): 5613-5617.
[5] Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging[J]. Cold Spring Harbor Protocols, 2010, 2010(5): pdb. prot4947.
| Cas No. | 17466-45-4 | SDF | |
| Sinónimos | NSC 523214 | ||
| Chemical Name | phalloidin | ||
| Canonical SMILES | O[C@@H]1C[C@H](C(N[C@@H](C)C(N[C@@H](C(N[C@@H](C(N[C@@H]2C)=O)C[C@](CO)(C)O)=O)CC3=C(NC4=CC=CC=C34)SC[C@H]5NC([C@H]([C@H](C)O)NC2=O)=O)=O)=O)N(C5=O)C1 | ||
| Formula | C35H48N8O11S | M.Wt | 788.87 |
| Solubility | 10mg/mL in ethanol, or in DMSO, or in DMF , 0.5 mg/ml in warm water | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2676 mL | 6.3382 mL | 12.6764 mL |
| 5 mM | 253.5 μL | 1.2676 mL | 2.5353 mL |
| 10 mM | 126.8 μL | 633.8 μL | 1.2676 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)