Phalloidin (Synonyms: NSC 523214) |
| Catalog No.GC15692 |
Phalloidin is a mushroom-derived toxin that can be conjugated to fluorescent dyes to label cytoskeletal F-actin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17466-45-4
Sample solution is provided at 25 µL, 10mM.
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Phalloidin is a mushroom-derived toxin that can be conjugated to fluorescent dyes to label cytoskeletal F-actin[1]. Phalloidin is a highly selective bicyclic peptide that works by binding and stabilizing F-actin, effectively preventing actin filament depolymerization[2]. Phalloidin derivatives containing fluorescent tags are widely used to visualize F-actin in biomedical research[3]. Phalloidin interferes with cell motility and growth[4]. Phalloidin is not suitable for methanol-fixed cells, probably due to the disruption of actin filament integrity, but it is suitable for cells fixed in 0.2% glutaraldehyde in PBS[5]. For RITC-Phalloidin, the maximum excitation wavelength is 495nm (or 550nm) and the maximum emission wavelength is 520nm[5].
References:
[1] DesMarais V, Eddy R J, Sharma V P, et al. Optimizing leading edge F-actin labeling using multiple actin probes, fixation methods and imaging modalities[J]. BioTechniques, 2019, 66(3): 113-119.
[2] Gübeli R J, Bertoldo D, Shimada K, et al. In Vitro-Evolved Peptides Bind Monomeric Actin and Mimic Actin-Binding Protein Thymosin-β4[J]. ACS Chemical Biology, 2021, 16(5): 820-828.
[3] Romani M, Auwerx J. Phalloidin staining of actin filaments for visualization of muscle fibers in Caenorhabditis elegans[J]. Bio-protocol, 2021, 11(19): e4183-e4183.
[4] Wehland J, Osborn M, Weber K. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth[J]. Proceedings of the National Academy of Sciences, 1977, 74(12): 5613-5617.
[5] Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging[J]. Cold Spring Harbor Protocols, 2010, 2010(5): pdb. prot4947.
| Cas No. | 17466-45-4 | SDF | |
| Synonyms | NSC 523214 | ||
| Chemical Name | phalloidin | ||
| Canonical SMILES | O[C@@H]1C[C@H](C(N[C@@H](C)C(N[C@@H](C(N[C@@H](C(N[C@@H]2C)=O)C[C@](CO)(C)O)=O)CC3=C(NC4=CC=CC=C34)SC[C@H]5NC([C@H]([C@H](C)O)NC2=O)=O)=O)=O)N(C5=O)C1 | ||
| Formula | C35H48N8O11S | M.Wt | 788.87 |
| Solubility | 10mg/mL in ethanol, or in DMSO, or in DMF , 0.5 mg/ml in warm water | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.2676 mL | 6.3382 mL | 12.6764 mL |
| 5 mM | 0.2535 mL | 1.2676 mL | 2.5353 mL |
| 10 mM | 0.1268 mL | 0.6338 mL | 1.2676 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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