Phalloidin (Synonyms: NSC 523214) |
| Catalog No.GC15692 |
Phalloidin binds F-actin and stabilizes actin fibers.
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Cas No.: 17466-45-4
Sample solution is provided at 25 µL, 10mM.
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Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome(λex=495 nm, λem=520 nm).
Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS. For fluorescein isothiocyanate (FITC)-Phalloidin (or rhodamine isothiocyanate [RITC]-Phalloidin), the excitation wavelength maximum will be near 495 nm (or 550 nm) and the emission wavelength maximum will be ~520 nm based on H2O at pH 8.0 as the solvent (575 nm with methanol as the solvent). The emission spectrum of fluorescein can depend on the environmental pH and/or ionic strength[1].
References:
[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.
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