DOTAP chloride (Synonyms: 1,2-Dioleoyl-3-trimethylammoniumpropane (chloride)) |
Catalog No.GC41811 |
Le chlorure de DOTAP est utilisé dans les formulations de liposomes pour transférer l'ADN plasmidique dans les cellules eucaryotes en culture.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 132172-61-3
Sample solution is provided at 25 µL, 10mM.
DOTAP chloride is a liposome formulations have been used to transfect plasmid DNA into eukaryotic cells in culture [1]. The synthetic cationic amphiphile DOTAP chloride has been shown to mediate uptake of plasmid also in the presence of a culture medium containing serum. It is metabolized in the cells and has proven to be less cytotoxic than other amphiphiles [2]. DOTAP chloride has also been used successfully for internalization of an 18-mer DNA oligonucleotide, and it was shown in these experiments to protect the oligonucleotide from degradation by nucleases in serum and cells [3].
References:
[1]. Haukenes G, Szilvay A M, Brokstad K A, et al. Labeling of RNA transcripts of eukaryotic cells in culture with BrUTP using a liposome transfection reagent (DOTAP®)[J]. Biotechniques, 1997, 22(2): 308-312.
[2]. Leventis R, Silvius J R. Interactions of mammalian cells with lipid dispersions containing novel metabolizable cationic amphiphiles[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1990, 1023(1): 124-132.
[3]. Capaccioli S, Dipasquale G, Mini E, et al. Cationic lipids improve antisense oligonucleotide uptake and prevent degradation in cultured cells and in human serum[J]. Biochemical and biophysical research communications, 1993, 197(2): 818-825.
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