DOTAP chloride (Synonyms: 1,2-Dioleoyl-3-trimethylammoniumpropane (chloride)) |
Catalog No.GC41811 |
DOTAP chloride is a liposome formulations have been used to transfect plasmid DNA into eukaryotic cells in culture .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 132172-61-3
Sample solution is provided at 25 µL, 10mM.
DOTAP chloride is a liposome formulations have been used to transfect plasmid DNA into eukaryotic cells in culture [1]. The synthetic cationic amphiphile DOTAP chloride has been shown to mediate uptake of plasmid also in the presence of a culture medium containing serum. It is metabolized in the cells and has proven to be less cytotoxic than other amphiphiles [2]. DOTAP chloride has also been used successfully for internalization of an 18-mer DNA oligonucleotide, and it was shown in these experiments to protect the oligonucleotide from degradation by nucleases in serum and cells [3].
References:
[1]. Haukenes G, Szilvay A M, Brokstad K A, et al. Labeling of RNA transcripts of eukaryotic cells in culture with BrUTP using a liposome transfection reagent (DOTAP®)[J]. Biotechniques, 1997, 22(2): 308-312.
[2]. Leventis R, Silvius J R. Interactions of mammalian cells with lipid dispersions containing novel metabolizable cationic amphiphiles[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1990, 1023(1): 124-132.
[3]. Capaccioli S, Dipasquale G, Mini E, et al. Cationic lipids improve antisense oligonucleotide uptake and prevent degradation in cultured cells and in human serum[J]. Biochemical and biophysical research communications, 1993, 197(2): 818-825.
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