740 Y-P (Synonyms: 740YPDGFR; PDGFR 740Y-P) |
| Catalog No.GC16157 |
Le 740 Y-P est un activateur de PI3K avec une concentration de 20 μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1236188-16-1
Sample solution is provided at 25 µL, 10mM.
Le 740 Y-P est un activateur de PI3K avec une concentration de 20 μM[1]. La PI3K (phosphoinositide 3-kinases, PI3Ks) est une enzyme qui joue un rôle crucial dans les processus cellulaires fondamentaux tels que le trafic vésiculaire, la dégranulation cellulaire, la migration cellulaire et le transporteur de glucose. Il a été rapporté qu'une surexpression de la PI3K est corrélée avec divers types de cancers [2]. Le 740 Y-P est un puissant activateur de la PI3K et joue un rôle important dans la voie de signalisation PI3K/AKT. Lors de tests sur des cellules de mélanome humain MNT-1, un traitement avec 20 μM de 740 Y-P pendant 24 heures a significativement réduit le nombre de vacuoles positives pour M6PR induites par le saccharose en activant la PI3K [1]. Dans les cellules granulaires cérébelleuses en situation de privation de sérum, le traitement avec 740 Y-P a réduit le taux de mortalité cellulaire en se liant à p85, ce qui est corrélé au processus de phosphorylation de l'Akt dépendant de la PI 3-kinase [3].
References:
[1]. Bin, B.H., et al., Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One, 2014. 9(8): p. e105965.
[2]. Kong, B., et al., A subset of metastatic pancreatic ductal adenocarcinomas depends quantitatively on oncogenic Kras/Mek/Erk-induced hyperactive mTOR signalling. Gut, 2015.
[3]. Williams, E.J. and P. Doherty, Evidence for and against a pivotal role of PI 3-kinase in a neuronal cell survival pathway. Mol Cell Neurosci, 1999. 13(4): p. 272-80.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules de mélanome humain MNT-1 |
Méthode de préparation | Solubilité limitée. Conseils généraux pour obtenir une concentration plus élevée : chauffer le tube à 37 °C pendant 10 minutes et/ou l’agiter dans un bain ultrasonique pendant un certain temps. La solution stock peut être conservée à une température inférieure à -20 °C pendant plusieurs mois. |
Conditions de réaction | 24 h |
Domaines d'application | Le 740 Y-P induit la génération de puncta positifs pour le M6PR, similaire aux vacuoles induites par le saccharose. De plus, le 740 Y-P réduit de manière significative le nombre de vacuoles positives pour le M6PR déclenchées par le traitement au saccharose. |
Références : 1. Bin BH, Bhin J, Yang SH et al. Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One. 2014 Aug 29;9(8):e105965. | |
| Cas No. | 1236188-16-1 | SDF | |
| Synonymes | 740YPDGFR; PDGFR 740Y-P | ||
| Formula | C141H222N43O39PS3 | M.Wt | 3270.72 |
| Solubility | ≥ 163.536mg/mL in DMSO | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3057 mL | 1.5287 mL | 3.0574 mL |
| 5 mM | 0.0611 mL | 0.3057 mL | 0.6115 mL |
| 10 mM | 0.0306 mL | 0.1529 mL | 0.3057 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Fu Fang Gang Liu liquid (FFGL) inhibits the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. D Cells were treated with FFGL (80 μg/mL) with various concentrations of 740 Y-P for 48 h. Cell viability was measured using the cell-counting kit 8 assay.
Similarly, we treated HeLa cells with the PI3K activator 740 Y-P (GlpBio, Montclair,CA, USA) (0, 12.5, 25, 50, 100, 200 μg/mL) in combination with FFGL (80 μg/mL) for 48 h.
Bmc Complement Med 23.1 (2023): 128. PMID: 37081536 IF: 2.838 -
Related Biological Data
THC inhibits the activation of the PI3K/AKT/mTOR pathway. g-i. Next, the same cells were treated for 48 hours to 15 μM 740Y-P (PI3K activator) alone, 100 μM THC alone, or 15 μM 740Y-P plus 100 μM THC.
We ordered the 740Y-P from GlpBio Technology (Montclair, CA, USA).
Arch Clin Psychiat 50.6 (2023). IF: 0.9839
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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