9,10-Anthracenediyl-bis(methylene)dimalonic Acid (Synonyms: ABMDMA)

This example only provides a guideline, please modify it to suit your specific needs.

Application example: Determination of singlet oxygen using 9,10-Anthracenediyl-bis(methylene)dimalonic Acid (ADMA/ABMDMA)

Literature source: Juan Wu, et al. Photodynamic effect of curcumin on Vibrio parahaemolyticus. Photodiagnosis Photodyn Ther. 2016 Sep:15:34-9. doi: 10.1016/j.pdpdt.2016.05.004. Epub 2016 May 10.

Experimental method: Use ABMDMA as a chemical ¹O₂ probe to measure singlet oxygen production. The probe reacts irreversibly with ¹O₂, resulting in a decrease in the absorption intensity of ABMDMA at about 400 nm. Briefly, ABMDMA (final concentration 100 μM) was added to a solution of curcumin in PBS buffer (2 ml) to a final concentration of 10μM. The solution was then irradiated with 470 nm LED light for 20 min, and the absorption intensity of ABMDMA at 400 nm was recorded at given time intervals. ABMDMA and curcumin were irradiated respectively as control experiments. Three parallel experiments were set up for each sample, and the experiments were repeated at least three times.

Experimental results:

Decay curves of the absorption of ADMA as a function of irradiation time.

Precautions:

1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.

2) For your safety and health, please wear a lab coat and disposable gloves.