L-Albizziine (Synonyms: NSC 132089, L-α-amino-β-Ureidopropionic Acid, L-β-Ureidoalanine) |
Catalog No.GA23127
One-Click Copy Product Info
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L-Albizziine est un inhibiteur de 4-méthylèneglutaminase, sa valeur d'IC50 est 20mM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1483-07-4
Sample solution is provided at 25 µL, 10mM.
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L-Albizziine est un inhibiteur de 4-méthylèneglutaminase, sa valeur d'IC50 est 20mM [1]. L-Albizziine, un inhibiteur de la glutamate synthase, a fortement inhibé la conversion du 2-cétoglutarate dans les feuilles de maïs[2]. L-Albizziine inhibe l'activité de l'asparagine synthétase et a été largement utilisé pour réguler l'activation des lymphocytes T dans différents modèles cellulaires[3].
Le traitement in vitro avec L-Albizziine à 2mM pendant 72 heures a significativement réduit le niveau d'aspartate et augmenté l'expression de la protéine p53 dans les cellules HuCCT1[4]. Le traitement avec 4mM de L-Albizziine pendant 48 heures a inhibé la prolifération des cellules HCT116 p53+/+ et p53−/− et réduit le nombre de cellules[5]. Le traitement avec 10mM de L-Albizziine pendant 24h a perturbé la réplication du virus de la variole dans les fibroblastes primaires de la peau humaine sans affecter la viabilité cellulaire[6].
References:
[1] Ibrahim S A, Lea P J, Fowden L. Purification and properties of 4-methyleneglutaminase from the leaves of peanut (Arachis hypogaea)[J]. Phytochemistry, 1984, 23(8): 1545-1549.
[2] Harel E, Ne'Eman E. Alternative Routes for the Synthesis of 5-Aminolevulinic Acid in Maize Leaves: II. Formation from Glutamate[J]. Plant Physiology, 1983, 72(4): 1062-1067.
[3] Wu J, Li G, Li L, et al. Asparagine enhances LCK signalling to potentiate CD8+ T-cell activation and anti-tumour responses[J]. Nature cell biology, 2021, 23(1): 75-86.
[4] Chu Z, Zhang B, Zhou X, et al. A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma[J]. Molecular Therapy Nucleic Acids, 2023, 34.
[5] Deng L, Yao P, Li L, et al. p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival[J]. Nature communications, 2020, 11(1): 1755.
[6] Pant A, Cao S, Yang Z. Asparagine is a critical limiting metabolite for vaccinia virus protein synthesis during glutamine deprivation[J]. Journal of Virology, 2019, 93(13): 10.1128/jvi. 01834-18.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules HuCCT1 |
Méthode de préparation | Les cellules HuCCT1 transfectées avec FGFR2-AHCYL1 (FA Cho-HDO) (200nM) ont été cultivées dans du milieu RPMI-1640 avec ou sans L-Albizziine (2mM) ou Asn (0.3mM) pendant 72h, le milieu était complété par 10% de sérum de veau foetal (SVF) et 1% de pénicilline/streptomycine, et les cultures ont été incubées dans une atmosphère humidifiée avec 5% CO2. Les protéines cellulaires ont été extraites et soumises à une analyse par Western Blot en utilisant un tampon de lyse RIPA contenant des inhibiteurs de protéases/phosphatases. |
Conditions de réaction | 2mM; 72h |
Domaines d'application | Le traitement avec L-Albizziine a inhibé l'activité de l'asparagine synthétase et augmenté l'expression de la protéine p53 dans les cellules HuCCT1. |
References: | |
| Cas No. | 1483-07-4 | SDF | |
| Synonymes | NSC 132089, L-α-amino-β-Ureidopropionic Acid, L-β-Ureidoalanine | ||
| Formula | C4H9N3O3 | M.Wt | 147.13 |
| Solubility | 50mg/ml in water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.7967 mL | 33.9836 mL | 67.9671 mL |
| 5 mM | 1.3593 mL | 6.7967 mL | 13.5934 mL |
| 10 mM | 679.7 μL | 3.3984 mL | 6.7967 mL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)