L-Albizziine (Synonyms: NSC 132089, L-α-amino-β-Ureidopropionic Acid, L-β-Ureidoalanine) |
Catalog No.GA23127
One-Click Copy Product Info
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L-Albizziine is an inhibitor of 4-methyleneglutaminase, with an IC50 value of 20mM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1483-07-4
Sample solution is provided at 25 µL, 10mM.
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L-Albizziine is an inhibitor of 4-methyleneglutaminase, with an IC50 value of 20mM [1]. L-Albizziine, an inhibitor of glutamate synthase, strongly inhibited the conversion of 2-ketoglutarate in maize leaves[2]. L-Albizziine inhibits asparagine synthetase activity and has been widely used to regulate T-cell activation in different cell models[3].
In vitro, L-Albizziine treatment at 2mM for 72 hours significantly reduced aspartate levels and increased p53 protein expression in HuCCT1 cells[4]. Treatment with 4mM L-Albizziine for 48 hours inhibited p53+/+ and p53−/− HCT116 cell proliferation and reduced cell number[5]. Treatment with 10mM L-Albizziine for 24h impaired vaccinia virus replication in primary human foreskin fibroblasts without affecting cell viability[6].
References:
[1] Ibrahim S A, Lea P J, Fowden L. Purification and properties of 4-methyleneglutaminase from the leaves of peanut (Arachis hypogaea)[J]. Phytochemistry, 1984, 23(8): 1545-1549.
[2] Harel E, Ne'Eman E. Alternative Routes for the Synthesis of 5-Aminolevulinic Acid in Maize Leaves: II. Formation from Glutamate[J]. Plant Physiology, 1983, 72(4): 1062-1067.
[3] Wu J, Li G, Li L, et al. Asparagine enhances LCK signalling to potentiate CD8+ T-cell activation and anti-tumour responses[J]. Nature cell biology, 2021, 23(1): 75-86.
[4] Chu Z, Zhang B, Zhou X, et al. A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma[J]. Molecular Therapy Nucleic Acids, 2023, 34.
[5] Deng L, Yao P, Li L, et al. p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival[J]. Nature communications, 2020, 11(1): 1755.
[6] Pant A, Cao S, Yang Z. Asparagine is a critical limiting metabolite for vaccinia virus protein synthesis during glutamine deprivation[J]. Journal of Virology, 2019, 93(13): 10.1128/jvi. 01834-18.
| Cell experiment [1]: | |
Cell lines | HuCCT1 cells |
Preparation Method | HuCCT1 cells transfected with FGFR2-AHCYL1 (FA Cho-HDO) (200nM) were cultured in RPMI-1640 medium with or without L-Albizziine (2mM) or Asn (0.3mM) for 72h, medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and cultures were incubated in a humidified atmosphere with 5% CO2. Cellular proteins were extracted and subjected to Western blot analysis using RIPA lysis buffer containing protease/phosphatase inhibitors. |
Reaction Conditions | 2mM; 72h |
Applications | L-Albizziine treatment inhibited asparagine synthase activity and increased p53 protein expression in HuCCT1 cells. |
References: | |
| Cas No. | 1483-07-4 | SDF | |
| Synonyms | NSC 132089, L-α-amino-β-Ureidopropionic Acid, L-β-Ureidoalanine | ||
| Formula | C4H9N3O3 | M.Wt | 147.13 |
| Solubility | 50mg/ml in water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.7967 mL | 33.9836 mL | 67.9671 mL |
| 5 mM | 1.3593 mL | 6.7967 mL | 13.5934 mL |
| 10 mM | 679.7 μL | 3.3984 mL | 6.7967 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)