Janelia Fluor 585, SE (Synonyms: JF585, SE; JF585, NHS) |
| Catalog No.GC50443 |
Janelia Fluor 585, SE (JF585, SE) est un colorant fluorescent orange contenant un ester NHS qui peut être conjugué avec des groupes amine primaire.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1811539-88-4
Sample solution is provided at 25 µL, 10mM.
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Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining. To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)
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