Janelia Fluor 585, SE (Synonyms: JF585, SE; JF585, NHS) |
| Catalog No.GC50443 |
Janelia Fluor 585, SE(JF585, SE)는 1차 아민기와 결합할 수 있는 NHS 에스테르를 포함하는 주황색 형광 염료입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1811539-88-4
Sample solution is provided at 25 µL, 10mM.
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Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining. To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)
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