P53R3 |
| Catalog No.GC65961 |
P53R3 est un puissant réactivateur de p53 et restaure la liaison À l'ADN spécifique À la séquence des mutants de points chauds de p53, y compris p53R175H, p53R248W et p53R273H. P53R3 induit des effets antiprolifératifs dépendants de p53 avec une spécificité beaucoup plus élevée que PRIMA-1. P53R3 améliore le recrutement de p53 et p53M237I de type sauvage sur plusieurs promoteurs de gènes cibles. P53R3 améliore fortement l'expression de l'ARNm, des protéines totales et de la surface cellulaire du récepteur de mort récepteur de mort 5 (DR5). Le P53R3 est utilisé pour la recherche sur le cancer.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 922150-12-7
Sample solution is provided at 25 µL, 10mM.
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P53R3 is a potent p53 reactivator and restores sequence-specific DNA binding of p53 hot spot mutants, including p53R175H, p53R248W and p53R273H. P53R3 induces p53-dependent antiproliferative effects with much higher specificity than PRIMA-1. P53R3 enhances the recruitment of wild-type p53 and p53M237I to several target gene promoters. P53R3 strongly enhances the mRNA, total protein and cell surface expression of the death receptor death receptor 5 (DR5). P53R3 is used for cancer research[1].
P53R3 (10 μg/ml; 24 hours; in the absence or presence of the unlabelled p53 consensus oligonucleotide) restores p53-specific DNA binding activity to p53R273H (a DNA contact mutant) and p53R175H (a structural mutant) in WiDr colon tumour cells harbouring p53R273H and KLE cells with p53R175H[1].
P53R3 (1-33 μg/ml; 24 hours) inhibits the proliferation of the LN-308 sublines expressing mutant p53 plasmids in a p53-dependent manner. The p53R175H-dependent effects are strong over a broad range of concentrations, but p53R273H-dependent effects are weaker and requires high concentrations of P53R3[1].
P53R3 induces p53R248W reactivation is more pronounced proliferation inhibition than observed with p53R273H. P53R3 does not exhibit cytotoxic effects even at concentrations close to its solubility limit (33 μg/ml)[1].
P53R3 (33 μg/ml; 18 hours) induces a strong decrease in S phase cells and a G0/G1 cell cycle arrest in LN-308 p53R175H and LN-308 p53R273H cells. But it does not affect cell cycle distribution of LN-308 p53R248W cells[1].
Cell Viability Assay[1]
| Cell Line: | p53 null LN-308 human glioma cells with a control plasmid or plasmids encoding the mutants p53R175H, p53R248W and p53R273H |
| Concentration: | 1-33 μg/mL |
| Incubation Time: | 24 hours |
| Result: | Induced p53-dependent and -independent antiproliferative and cytotoxic effects in vitro. |
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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