RBITC |
Catalog No.GC19883
One-Click Copy Product Info
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RBITC, ou isothiocyanate de Rhodamine B, est un colorant fluorescent qui est connu pour son groupe isothiocyanate qui peut se lier de manière covalante aux amines primaires des protéines et des biomoclécules.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 36877-69-7
Sample solution is provided at 25 µL, 10mM.
RBITC, ou isothiocyanate de Rhodamine B, est un colorant fluorescent qui est connu pour son groupe isothiocyanate qui peut se lier de manière covalante aux amines primaires des protéines et des biomoclécules[1][2]. RBITC possède une longueur d'onde d'excitation maximale de 562nm et d'émission de 582nm[3]. RBITC est principalement utilisé dans la biologie cellulaire[4], la biologie moléculaire, l'immunologie pour la localisation des protéines[5], la suivi de cellule[6], et la recherche des processus cellulaire[7].
References:
[1]. Weiss HR. Measurement of cerebral capillary perfusion with a fluorescent label. Microvasc Res. 1988 Sep;36(2):172-80. doi: 10.1016/0026-2862(88)90017-9. PMID: 3141746.
[2]. Yang T, Sun D, Xu P, et al. Stability of bovine serum albumin labelled by rhodamine B isothiocyanate[J]. Biomed. Res, 2017, 28(9): 3851-3854.
[3]. Poon CK, Tang O, Chen XM, et al. Fluorescent Labeling and Biodistribution of Latex Nanoparticles Formed by Surfactant-Free RAFT Emulsion Polymerization. Macromol Biosci. 2017 Oct;17(10). doi: 10.1002/mabi.201600366. Epub 2016 Dec 14. PMID: 27973756.
[4]. Butcher EC, Weissman IL. Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects. J Immunol Methods. 1980;37(2):97-108. doi: 10.1016/0022-1759(80)90195-7. PMID: 7003013.
[5]. Nigen M, Croguennec T, Madec MN, et al. Apo alpha-lactalbumin and lysozyme are colocalized in their subsequently formed spherical supramolecular assembly. FEBS J. 2007 Dec;274(23):6085-93. doi: 10.1111/j.1742-4658.2007.06130.x. Epub 2007 Oct 26. PMID: 17970750.
[6]. Luo Y, Cai X, Li H, et al. Hyaluronic Acid-Modified Multifunctional Q-Graphene for Targeted Killing of Drug-Resistant Lung Cancer Cells. ACS Appl Mater Interfaces. 2016 Feb 17;8(6):4048-55. doi: 10.1021/acsami.5b11471. Epub 2016 Feb 3. PMID: 26785717.
[7]. Shi H, He X, Wang K, et al. Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)-based bioprobes conjugated to Annexin V for apoptosis detection and imaging. Nanomedicine. 2007 Dec;3(4):266-72. doi: 10.1016/j.nano.2007.08.004. Epub 2007 Nov 7. PMID: 17988954.
Le procotol expérimental de marquage des protéines par RBITC:
1.Préparation de la solution RBITC : Dissoudre RBITC à 10mg/mL dans DMSO avant l'utilisation. Mélanger soigneusement.
2.Préparation de la solution de protéine : Dissoudre la protéine de cible dans un tampon 0.15M NaCl, 0.1M phosphate de 0,1M sodium à pH 7,2 pour former 10mg/mL solution de protéine.
3.Réaction de marquage : Transférer la solution RBITC dans la solution de protéine au ratio molaire de 5:1 et mélanger doucement le mélange en obscurité à 4°C pendant 12h.
4.Dialyse : Retirer RBITC non - réagi par dialyse ou par la colonne d'élimination de colorant.
5.Après la dialyse, collecter la solution de protéine marquée, déterminer sa concentration, et stocker la protéine RBITC-marquée à 4°C, loin de lumière, jusqu'à l'utilisation.
Ce protocol ne fournit qu'une ligne de guide et doit être modifié selon vos besoins spécifiques.
[1]. Yang T, Sun D, Xu P, et al. Stability of bovine serum albumin labelled by rhodamine B isothiocyanate[J]. Biomed. Res, 2017, 28(9): 3851-3854.
| Cas No. | 36877-69-7 | SDF | |
| Formula | C29H30ClN3O3S | M.Wt | 536.08 |
| Solubility | Soluble in methanol (1%). | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8654 mL | 9.327 mL | 18.6539 mL |
| 5 mM | 373.1 μL | 1.8654 mL | 3.7308 mL |
| 10 mM | 186.5 μL | 932.7 μL | 1.8654 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)