RBITC |
Catalog No.GC19883
One-Click Copy Product Info
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RBITC또는 로다민 B 이소티오시안산은 단백질과 생물 분자에 있는 일차 아민에 공가결합할 수 있는 이소티오시안산 기를 가진 형광 염료로 알려져 있다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 36877-69-7
Sample solution is provided at 25 µL, 10mM.
RBITC또는 로다민 B 이소티오시안산은 단백질과 생물 분자에 있는 일차 아민에 공가결합할 수 있는 이소티오시안산 기를 가진 형광 염료로 알려져 있다. RBITC는 최대 흡수 파장이 562nm이고 방출 파장은 582nm이다[3]. RBITC는 주로 세포 생물학[4], 분자 생물학, 면역학에서 단백질 정위[5], 세포 추적[6], 세포 과정 연구[7]에 사용된다.
References:
[1]. Weiss HR. Measurement of cerebral capillary perfusion with a fluorescent label. Microvasc Res. 1988 Sep;36(2):172-80. doi: 10.1016/0026-2862(88)90017-9. PMID: 3141746.
[2]. Yang T, Sun D, Xu P, et al. Stability of bovine serum albumin labelled by rhodamine B isothiocyanate[J]. Biomed. Res, 2017, 28(9): 3851-3854.
[3]. Poon CK, Tang O, Chen XM, et al. Fluorescent Labeling and Biodistribution of Latex Nanoparticles Formed by Surfactant-Free RAFT Emulsion Polymerization. Macromol Biosci. 2017 Oct;17(10). doi: 10.1002/mabi.201600366. Epub 2016 Dec 14. PMID: 27973756.
[4]. Butcher EC, Weissman IL. Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocyanate. I. Technical aspects. J Immunol Methods. 1980;37(2):97-108. doi: 10.1016/0022-1759(80)90195-7. PMID: 7003013.
[5]. Nigen M, Croguennec T, Madec MN, et al. Apo alpha-lactalbumin and lysozyme are colocalized in their subsequently formed spherical supramolecular assembly. FEBS J. 2007 Dec;274(23):6085-93. doi: 10.1111/j.1742-4658.2007.06130.x. Epub 2007 Oct 26. PMID: 17970750.
[6]. Luo Y, Cai X, Li H, et al. Hyaluronic Acid-Modified Multifunctional Q-Graphene for Targeted Killing of Drug-Resistant Lung Cancer Cells. ACS Appl Mater Interfaces. 2016 Feb 17;8(6):4048-55. doi: 10.1021/acsami.5b11471. Epub 2016 Feb 3. PMID: 26785717.
[7]. Shi H, He X, Wang K, et al. Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)-based bioprobes conjugated to Annexin V for apoptosis detection and imaging. Nanomedicine. 2007 Dec;3(4):266-72. doi: 10.1016/j.nano.2007.08.004. Epub 2007 Nov 7. PMID: 17988954.
RBITC로 단백질을 표지하는 실험 프로토콜:
1.RBITC 용액 준비: 사용 전 DMSO에 RBITC를 10mg/mL로 녹여 완전히 혼합합니다.
2.단백질 용액 준비: 표적 단백질을 pH 7.2의 0.1M 나트리움 인산염, 0.15M NaCl 버퍼에 녹여 10mg/mL 단백질 용액을 제조합니다.
3.표지 반응: RBITC 용액을 단백질 용액에 5:1의 몰비로 옮겨 4°C의 어두운 곳에서 12시간 동안 부드럽게 저어 줍니다.
4. 투석: 반응하지 않은 RBITC를 투석하거나 염료 제거 칼럼으로 사용하고 제거합니다. 5. 투석 후 표지된 단백질 용액을 수집하고 그 농도를 측정한 후, RBITC로 표지된 단백질을 사용할 때까지 4°C에서 빛을 차단하고 보관합니다.
이 프로토콜은 단지 가이드라인을 제공하고 필요에 따라 수정해야 합니다.
[1]. Yang T, Sun D, Xu P, et al. Stability of bovine serum albumin labelled by rhodamine B isothiocyanate[J]. Biomed. Res, 2017, 28(9): 3851-3854.
| Cas No. | 36877-69-7 | SDF | |
| Formula | C29H30ClN3O3S | M.Wt | 536.08 |
| Solubility | Soluble in methanol (1%). | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8654 mL | 9.327 mL | 18.6539 mL |
| 5 mM | 373.1 μL | 1.8654 mL | 3.7308 mL |
| 10 mM | 186.5 μL | 932.7 μL | 1.8654 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)