2-5A TEA (Synonyms: 2′,5′-ApApA TEA; 2′,5′-trioligoadenylate TEA; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine TEA) |
カタログ番号GC81654
One-Click Copy Product Info
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2-5A TEA (2′,5′-ApApA TEA) is a 5'-triphosphorylated (2',5') oligoadenylate.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 72351-33-8
Sample solution is provided at 25 µL, 10mM.
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In Vivo, 2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) TEA complexed with lipofectamine significantly inhibits MC38 colorectal carcinoma tumor growth in wild-type C57BL/6J mice[3]. 2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) TEA complexed with lipofectamine significantly inhibits B16 melanoma tumor growth in wild-type C57BL/6J mice[3]. 2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) TEA complexed with lipofectamine significantly inhibits sgRnasel MC38 colorectal carcinoma tumor growth in wild-type C57BL/6J mice[3]. 2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) TEA complexed with lipofectamine does not inhibit MC38 colorectal carcinoma tumor growth in RnaseI−/− mice[3].
In Vitro, 2-5A (1 μM; 4 h) TEA is imported by human HEK293T cells and BJ1 fibroblasts to activate RNase L-dependent IFN/ISG induction, while mouse MEFs, MC38 cells, BMDMs, human A549 cells, and THP-1 monocytes do not exhibit robust 2-5A TEA import activity[3].
References:
[1]. Barnard DL, et al. 2-5A-DNA conjugate inhibition of respiratory syncytial virus replication: effects of oligonucleotide structure modifications and RNA target site selection. Antiviral research. 1999 Apr;41(3):119-34.
[2]. Wang S, et al. 2-5A is an immunotransmitter that fuels RNase L immunity. Immunity. 2025 Apr 08;58(4):770-772.
[3]. Huai W, et al. OAS cross-activates RNase L intercellularly through cell-to-cell transfer of 2-5A to spread innate immunity. Immunity. 2025 Apr 08;58(4):797-810.e6.
[4]. Kalinichenko EN, et al. 3'-Fluoro-3'-deoxy analogs of 2-5A 5'-monophosphate: binding to 2-5A-dependent endoribonuclease and susceptibility to (2'-5')phosphodiesterase degradation. Biochemical and biophysical research communications. 1990 Feb 28;167(1):20-6.
| Cas No. | 72351-33-8 | SDF | |
| 同義語 | 2′,5′-ApApA TEA; 2′,5′-trioligoadenylate TEA; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine TEA | ||
| Formula | C30H40N15O25P5·xC6H15N | M.Wt | 1165.59 |
| 溶解度 | Storage | Store at -20°C | |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 857.9 μL | 4.2897 mL | 8.5793 mL |
| 5 mM | 171.6 μL | 857.9 μL | 1.7159 mL |
| 10 mM | 85.8 μL | 429 μL | 857.9 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)