AIZin2 |
カタログ番号GC92069
One-Click Copy Product Info
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AIZin2は亜鉛結合蛋白質のための条件標識化合物であり、亜鉛配位の三歯ジアピリジンアミン基、求電反応性アシルイミダゾール部分及びフルオレセインジアセテートを含有する。
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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AIZin2 is a conditional labeling compound for zinc-binding proteins that contains a zinc-coordinating tridentate dipicolylamine group, an electrophile-reactive acyl imidazole moiety, and fluorescein diacetate.1 It is attached to proteins via an electrophilic attack on the acyl imidazole moiety when coordinated with protein-associated zinc. AIZin2 has been used to label zinc-binding proteins in HeLa cells for fluorescent detection and for LC-MS/MS proteomic experiments. It displays an emission at 559 nm upon excitation at 473 nm.
References:
[1]. Miki, T., Awad, M., Nishikawa, Y., et al.A conditional proteomics approach to identify proteins involved in zinc homeostasisNat. Methods13(11)931-937(2016).
| Cas No. | SDF | ||
| Chemical Name | 5-((2-(2-((4-((bis(pyridin-2-ylmethyl)amino)methyl)-1H-imidazole-1-carbonyl)oxy)ethoxy)ethyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate | ||
| Canonical SMILES | O=C(OCCOCCNC(C1=CC(C(OC23C4=C(C=C(OC(C)=O)C=C4)OC5=CC(OC(C)=O)=CC=C53)=O)=C2C=C1)=O)N(C=N6)C=C6CN(CC7=NC=CC=C7)CC8=NC=CC=C8 | ||
| Formula | C46H40N6O11 | M.Wt | 852.8 |
| 溶解度 | Storage | -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1726 mL | 5.863 mL | 11.7261 mL |
| 5 mM | 234.5 μL | 1.1726 mL | 2.3452 mL |
| 10 mM | 117.3 μL | 586.3 μL | 1.1726 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)