Amidepsine A (Synonyms: FO-2942A) |
カタログ番号GC11675
One-Click Copy Product Info
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アミデプシン A は、Humicola sp. の培養液から分離された真菌代謝産物です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 169181-28-6
Sample solution is provided at 25 µL, 10mM.
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Amidepsine A is a fungal metabolite isolated from the culture broth of Humicola sp FO-2942. Amidepsine A is an inhibitor of dylglycerol acyltransferase (DGAT).
Dylglycerol acyltransferase (DGAT) has been involved in catalyzing the formation of triglycerides from diacylglycerol and Acyl-CoA. The reaction is considered the terminal and only committed step in triglyceride synthesis and is essential for the formation of adipose tissue. There are two isozymes of DGAT have been identified: DGAT1 and DGAT2. DGAT-1 deficient mice are lean and resistant to the development of diet-induced obesity or insulin resistance [1]. DGAT2-/- mice demonstrate reduced triglyceride levels and suffer from skin barrier abnormalities [2].
FO-2942 inhibited DGAT activity with an IC50 of 10.2 μM in rat liver microsomes. FO-2942 inhibited triacylglycerol formation in Raji cells with the IC50 value of 15.5 μM [1].
References:
[1] Tomoda H, Tabata N, Ito M, et al. Amidepsines, inhibitors of diacylglycerol acyltransferase produced by Humicola sp. FO-2942[J]. The Journal of antibiotics, 1995, 48(9): 942-947.
[2] Smith S J, Cases S, Jensen D R, et al. Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat[J]. Nature genetics, 2000, 25(1): 87-90.
[3] Stone S J, Myers H M, Watkins S M, et al. Lipopenia and skin barrier abnormalities in DGAT2-deficient mice[J]. Journal of Biological chemistry, 2004, 279(12): 11767-11776.
| Cas No. | 169181-28-6 | SDF | |
| 同義語 | FO-2942A | ||
| Chemical Name | 2,4-dimethoxy-6-methyl-benzoic acid, 4-[[4-[[(1-carboxyethyl)amino]carbonyl]-3-hydroxy-5-methylphenoxy]carbonyl]-3-hydroxy-5-methylphenyl ester | ||
| Canonical SMILES | OC1=C(C(NC(C)C(O)=O)=O)C(C)=CC(OC(C2=C(O)C=C(OC(C3=C(C)C=C(OC)C=C3OC)=O)C=C2C)=O)=C1 | ||
| Formula | C29H29NO11 | M.Wt | 567.5 |
| 溶解度 | DMSO : 5.79 mg/mL (10.20 mM; Need ultrasonic and warming) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7621 mL | 8.8106 mL | 17.6211 mL |
| 5 mM | 352.4 μL | 1.7621 mL | 3.5242 mL |
| 10 mM | 176.2 μL | 881.1 μL | 1.7621 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)